Recent Advances in Double-Strand Break-Free Kilobase-Scale Genome Editing Technologies.
Recent Advances in Double-Strand Break-Free Kilobase-Scale Genome Editing Technologies.
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DOI：
10.1021/acs.biochem.2c00311
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发表时间：
2022-09
期刊：
影响因子：
2.9
通讯作者：
Connor J. Tou;B. Kleinstiver
中科院分区：
文献类型：
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作者：
Connor J. Tou;B. Kleinstiver
基因组编辑方法已经改变了在离体和体内环境中对基因组进行用户定义的改变的能力。尽管允许安装核苷酸水平变化的技术得到了大量发展，但直到最近，通过独立于DNA双链断裂（DSB）的技术进行的大规模序列编辑仍然很少探索。在这里，我们回顾了最近的进展对DSB的自由技术，使酶规模的修改，包括插入，删除，倒位，替换，和其他。这些技术为用户提供了新的功能，同时通过从小的突变特异性编辑转向更普遍的酶规模方法，为简化假定的治疗策略提供了希望。
Genome editing approaches have transformed the ability to make user-defined changes to genomes in both ex vivo and in vivo contexts. Despite the abundant development of technologies that permit the installation of nucleotide-level changes, until recently, larger-scale sequence edits via technologies independent of DNA double-strand breaks (DSBs) had remained less explored. Here, we review recent advances toward DSB-free technologies that enable kilobase-scale modifications including insertions, deletions, inversions, replacements, and others. These technologies provide new capabilities for users, while offering hope for the simplification of putative therapeutic strategies by moving away from small mutation-specific edits and toward more generalizable kilobase-scale approaches.