Multipotency of clonal cells derived from swine periodontal ligament and differential regulation by fibroblast growth factor and bone morphogenetic protein
Multipotency of clonal cells derived from swine periodontal ligament and differential regulation by fibroblast growth factor and bone morphogenetic protein
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DOI：
10.1111/j.1600-0765.2008.01140.x
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发表时间：
2009-04-01
影响因子：
3.5
通讯作者：
Furuichi, Y.
中科院分区：
文献类型：
--
作者：
Shirai, K.;Ishisaki, A.;Furuichi, Y.
血液供应对于受损或丢失的牙周膜（PDL）组织的再生是必不可少的。来源于PDL的细胞的间充质干细胞样活性已经通过它们形成纤维和骨组织以及牙骨质的能力来鉴定。然而，这些细胞是否具有构建血管毛细血管网络的能力仍有待澄清。本研究评估了来源于PDL的细胞构建血管样结构的潜力，并检查了生长因子如何控制细胞的多能性。猪PDL成纤维细胞系TesPDL 3构建血管样结构的能力在自组装肽支架PuraMatrix（TM）上和中进行了评估。此外，在I型胶原包被的塑料板上评价细胞形成矿化结节的能力。在某些情况下，向这些培养物中加入成纤维细胞生长因子（FGF）-2和骨形态发生蛋白（BMP）-2。通过RT-PCR、Western blotting和免疫荧光分析检测细胞中血管和成骨细胞特异性标志物的表达情况，结果显示，TesPDL 3细胞不仅在BMP-2刺激下形成矿化结节，而且在FGF-2刺激下形成管状结构。有趣的是，FGF-2抑制BMP-2诱导的矿化结节的形成。相反，BMP-2抑制FGF-2诱导的管状结构的形成。牙周膜韧带成纤维细胞不仅具有分化成骨细胞的潜力，而且还具有分化成血管细胞系的潜力。BMP-2和FGF-2共同调控细胞的命运。
A blood supply is indispensable for the regeneration of damaged or lost periodontal ligament (PDL) tissue. Mesenchymal stem cell-like activity of cells derived from the PDL has been identified by their capacity to form fibrous and osseous tissue and cementum. However, it remains to be clarified whether the cells have an ability to build the capillary network of blood vessels. This study evaluated the potential of cells derived from the PDL to construct a blood vessel-like structure and examined how growth factors controlled the multipotency of the cells.The ability of a swine PDL fibroblast cell line, TesPDL3, to construct a blood vessel-like structure was evaluated on and in the self-assembling peptide scaffold, PuraMatrix(TM). In addition, the ability of the cells to form mineralized nodules was evaluated on type I collagen-coated plastic plates. In some cases, fibroblast growth factor (FGF)-2 and bone morphogenetic protein (BMP)-2 were added to these cultures. The status of the expression of vascular and osteoblastic cell-specific markers in the cells was evaluated using reverse transcriptase-polymerase chain reaction (RT-PCR), Western blotting and immunofluorescence analyses.The TesPDL3 cells not only formed mineralized nodules in response to BMP-2 stimulation but also constructed tube-like structures in response to FGF-2 stimulation. Intriguingly, FGF-2 inhibited the BMP-2-induced formation of mineralized nodules. Conversely, BMP-2 inhibited the FGF-2-induced formation of tube-like structures.Periodontal ligament fibroblasts have the potential to differentiate not only into osteoblastic but also into vascular cell lineages. The destiny of the cells was reciprocally regulated by BMP-2 and FGF-2.