Analysis of epstein-Barr virus (EBV) of P3HR-1: isolation of EBV with EBNA induction ability in human cord lymphocytes and without EA induction ability in Raji cells.
Analysis of epstein-Barr virus (EBV) of P3HR-1: isolation of EBV with EBNA induction ability in human cord lymphocytes and without EA induction ability in Raji cells.
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P3HR-1 的 epstein-Barr 病毒 (EBV) 分析：分离出在人脐带淋巴细胞中具有 EBNA 诱导能力且在 Raji 细胞中不具有 EA 诱导能力的 EBV。
DOI：
10.1016/0042-6822(82)90098-8
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发表时间：
1982
期刊：
影响因子：
3.7
通讯作者：
Nonoyama,M
中科院分区：
文献类型：
--
作者：
Yano,S;Tanaka,A;Takada,K;Fujiwara,S;Osato,T;Nonoyama,M
P3 HR-1细胞的亚系通过在非EBV生产性条件下细胞的延长（超过1年）增殖，随后进行细胞克隆程序来分离。如此获得的克隆细胞，命名为DHR 1，当回到EBV-生产条件时产生EBV。病毒DNA的限制性内切酶分析表明，DHR 1 EBV由明显同质的EBV群体组成，与HR-1 EBV相比，它显示出相似但不相同的基因组结构。DHR 1 EBV的特征性生物学特性包括在人脐带淋巴细胞中诱导EBV相关核抗原（EBNA）的能力和在Raji细胞中不能诱导EBV相关早期抗原（EA）的能力。这些与亲本HR-1 EBV的行为形成鲜明对比。因此，P3 HR-1培养物在一段时间的非生产性后重新启动了病毒的生产，其具有与原始HR-1病毒可区分的明显同质且独特的EBV群体。
A subline of P3HR-1 cells was isolated through a prolonged (over 1 year) propagation of the cells at a non-EBV-productive condition followed by cell cloning procedures. Cloned cells thus obtained, designated DHR1, produced EBV when brought back to the EBV-productive condition. Restriction enzyme analysis of the viral DNA revealed that DHR1 EBV is composed of an apparently homogeneous EBV population, and it displays a similar but not identical genome organization compared with HR-1 EBV. The characteristic biological properties of DHR1 EBV included the ability to induce EBV-associated nuclear antigen (EBNA) in human cord lymphocytes and the inability to induce EBV-associated early antigen (EA) in Raji cells. These are in striking contrast to the behavior of the parental HR-1 EBV. Thus, P3HR-1 cultures after a period of nonproductivity reinitiated the production of virus with an apparently homogeneous and unique population of EBV distinguishable from the original HR-1 virus.