Physicochemical Characterization of Nebulized Superparamagnetic Iron Oxide Nanoparticles (SPIONs)
Physicochemical Characterization of Nebulized Superparamagnetic Iron Oxide Nanoparticles (SPIONs)
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DOI：
10.1089/jamp.2013.1117
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发表时间：
2015-02-01
影响因子：
3.4
通讯作者：
Riediker, Michael
中科院分区：
文献类型：
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作者：
Graczyk, Halshka;Bryan, Louise C.;Riediker, Michael
背景：气溶胶介导的纳米治疗药物向肺部的输送已成为治疗和预防肺部疾病的一种有前途的替代方案。超顺磁性氧化铁纳米颗粒（SPION）由于其生物相容性和磁性而在此类应用中引起了极大的关注。然而，关于用作治疗气雾剂的雾化 SPION 的特性尚缺乏信息。为了满足这一需求，我们对雾化 Rienso 进行了物理化学表征，这是一种基于 SPION 的制剂，用于静脉注射治疗贫血。方法：用单喷射雾化器雾化四种不同浓度的 SPION 悬浮液。通过透射电子显微镜 (TEM)、光子相关光谱 (PCS) 和纳米颗粒跟踪分析 (NTA) 测量悬浮液中的粒径，通过扫描迁移率粒径分析仪 (SMPS) 测量气溶胶中的粒径。 结果：通过 TEM、PCS 和 NTA 测量的悬浮液中的平均粒径分别为 9 +/- 2 nm、27 +/- 7 nm 和 56 +/- 10 nm。悬浮液中的颗粒尺寸在雾化过程之前和之后保持相同。然而，在撞击器中收集气溶胶后，根据 NTA 测量，悬浮颗粒尺寸增加至 159 +/- 46 nm。气溶胶颗粒浓度随着悬浮液浓度的增加而线性增加，并且通过 SMPS 测量，空气动力学直径在 75 nm 左右保持相对稳定。结论：我们证明，气溶胶中的总数和颗粒尺寸作为雾化器中初始浓度的函数进行调节。获得的数据标志着雾化 Rienso 的首次已知独立表征，因此提供了有关 Rienso 纳米粒子在气溶胶中行为的关键信息。本研究中获得的数据为现有文献中关于 SPION 悬浮液作为吸入气雾疗法的潜在应用增加了新的知识。
Background: Aerosol-mediated delivery of nano-based therapeutics to the lung has emerged as a promising alternative for treatment and prevention of lung diseases. Superparamagnetic iron oxide nanoparticles (SPIONs) have attracted significant attention for such applications due to their biocompatibility and magnetic properties. However, information is lacking about the characteristics of nebulized SPIONs for use as a therapeutic aerosol. To address this need, we conducted a physicochemical characterization of nebulized Rienso, a SPION-based formulation for intravenous treatment of anemia.Methods: Four different concentrations of SPION suspensions were nebulized with a one-jet nebulizer. Particle size was measured in suspension by transmission electron microscopy (TEM), photon correlation spectroscopy (PCS), and nanoparticle tracking analysis (NTA), and in the aerosol by a scanning mobility particle sizer (SMPS).Results: The average particle size in suspension as measured by TEM, PCS, and NTA was 9 +/- 2 nm, 27 +/- 7 nm, and 56 +/- 10 nm, respectively. The particle size in suspension remained the same before and after the nebulization process. However, after aerosol collection in an impinger, the suspended particle size increased to 159 +/- 46 nm as measured by NTA. The aerosol particle concentration increased linearly with increasing suspension concentration, and the aerodynamic diameter remained relatively stable at around 75 nm as measured by SMPS.Conclusions: We demonstrated that the total number and particle size in the aerosol were modulated as a function of the initial concentration in the nebulizer. The data obtained mark the first known independent characterization of nebulized Rienso and, as such, provide critical information on the behavior of Rienso nanoparticles in an aerosol. The data obtained in this study add new knowledge to the existing body of literature on potential applications of SPION suspensions as inhaled aerosol therapeutics.