Production of reactive oxygen species by spermatozoa undergoing cooling, freezing, and thawing
Production of reactive oxygen species by spermatozoa undergoing cooling, freezing, and thawing
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DOI：
10.1002/mrd.1052
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发表时间：
2001-08-01
影响因子：
2.5
通讯作者：
Gagnon, C
中科院分区：
文献类型：
--
作者：
Chatterjee, S;Gagnon, C
在本研究中，我们为牛精子冷冻保存过程中活性氧（ROS）的产生提供了证据。精子的冷却和解冻会导致超氧自由基的产生增加。尽管在精子从22-4度冷却期间一氧化氮的产生保持不变，但在解冻过程中观察到一氧化氮自由基的突然爆发。在冷冻/解冻精子中已经观察到脂质过氧化水平的增加，并且似乎与自旋标记研究检测到的精子膜流动性的减少有关。所提出的数据提供了强有力的证据，表明氧自由基在牛精子的冷冻和解冻过程中产生，并表明这些活性氧可能是冷冻保存后精子功能下降的原因。(C) 2001 Wiley-Liss, Inc。
In the present study, we provide evidence for the production of reactive oxygen species (ROS) during cryopreservation of bovine spermatozoa. Cooling and thawing of spermatozoa cause an increase in the generation of superoxide radicals. Although nitric oxide production remains unaltered during sperm cooling from 22-4 degreesC, a sudden burst of nitric oxide radicals is observed during thawing. Increase in lipid peroxidation levels have been observed in frozen/thawed spermatozoa and appears to be associated with a reduction in sperm membrane fluidity as detected by spin labeling studies. The data presented provide strong evidence that oxygen free radicals are produced during freezing and thawing of bovine spermatozoa and suggest that these reactive oxygen species may be a cause for the decrease in sperm function following cryopreservation. (C) 2001 Wiley-Liss, Inc.