Controlled release of bFGF loaded into electrospun core-shell fibrous membranes for use in guided tissue regeneration
Controlled release of bFGF loaded into electrospun core-shell fibrous membranes for use in guided tissue regeneration
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负载到电纺核壳纤维膜中的 bFGF 的受控释放用于引导组织再生
DOI：
10.1088/1748-605x/ab7979
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发表时间：
2020
影响因子：
4
通讯作者：
Zhang Wen
中科院分区：
文献类型：
--
作者：
Zhang Hualin;Wang Kairong;Gao Tong;Zhang Rui;Cai Zhuoyan;Liu Jinsong;Ma Hairong;Zhang Wen
本研究通过乳液静电纺丝将碱性成纤维细胞生长因子(BFGF)负载到聚乳酸-乙醇酸(PLGA)/羊毛角蛋白复合膜中，制备出具有核壳结构的复合膜，用于引导组织再生(GTR)。通过对复合膜的物理化学性质、体外释药性能和细胞毒性的评价，筛选出最佳的碱性成纤维细胞生长因子浓度。六组复合膜中的纤维厚度均匀，表面光滑，不呈现串珠结构。此外，六组复合膜中的纤维具有稳定的核壳结构，其中纤维的核心层包裹在碱性成纤维细胞生长因子周围，纤维的壳层由PLGA/羊毛角蛋白油相组成。与传统静电纺丝法制备的PLGA/羊毛角蛋白复合膜相比，乳液静电纺丝法制备的PLGA/羊毛角蛋白复合膜具有更高的吸水率和优异的亲水性。10μg碱性成纤维细胞生长因子复合膜对碱性成纤维细胞生长因子的包封率最高(97.3%±11.3%)。5组PLGA/羊毛角蛋白复合膜均能在28d内稳定、持续释放碱性成纤维细胞生长因子，对人牙周膜成纤维细胞的黏附、增殖和成骨分化有不同程度的促进作用。5组中，10μg碱性成纤维细胞生长因子复合膜组的细胞形态、增殖能力和成骨分化能力最好。本研究为进一步研究碱性成纤维细胞生长因子复合膜在GTR中的应用奠定了基础。
In this study, basic fibroblast growth factor (bFGF) was loaded into a poly (lactic-co-glycolic acid)(PLGA)/wool keratin composite membrane by emulsion electrospinning to prepare a composite membrane with a core–shell structure for guided tissue regeneration (GTR). The physicochemical properties, drug release performance in vitro and cytotoxicity of the composite membrane were evaluated to select the optimum concentration of bFGF. The fibers in the six groups of composite membranes were uniform in thickness, had a smooth surface, and did not exhibit a string bead structure. In addition, the fibers in the six groups of composite membranes had a stable core–shell structure, in which the core layer of the fibers was wrapped around bFGF and the shell layer of the fibers comprised the PLGA/wool keratin oil-phase component. Compared with the PLGA/wool keratin composite membrane prepared by traditional electrospinning, the composite membranes prepared by emulsion electrospinning had a higher water absorption rate and superior hydrophilicity. The bFGF encapsulation rate of the 10 μg bFGF composite membrane was the highest (97.3%±11.3%). Stable and sustained release of bFGF from the five groups of bFGF-loaded PLGA/wool keratin composite membranes can be maintained over 28 d. The five groups of PLGA/wool keratin composite membranes loaded with bFGF could promote the adhesion, proliferation and osteogenic differentiation of human periodontal ligament fibroblasts (hPLDFs) to varying degrees. Among the five groups, the cell morphology, proliferation ability and osteogenic differentiation ability of the 10 μg bFGF composite membrane group were the best. This study will serve as a foundation for further research on bFGF-loaded composite membranes for GTR applications.