DJ-1 mRNA anatomical localization and cell type identification in the mouse brain.
DJ-1 mRNA anatomical localization and cell type identification in the mouse brain.
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小鼠大脑中 DJ-1 mRNA 的解剖定位和细胞类型识别。
DOI：
10.1016/j.neulet.2009.09.024
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发表时间：
2009
影响因子：
2.5
通讯作者：
Chen,Linan
中科院分区：
文献类型：
--
作者：
Xie,Zhiguo;Zhuang,Xiaoxi;Chen,Linan
DJ-1突变导致家族性帕金森病（PD）。DJ-1在大脑中的表达模式仍然存在争议。在本研究中，我们使用DJ-1缺陷小鼠作为阴性对照，并检查DJ-1 mRNA在小鼠脑中的表达。在相同的切片上连续双标记，DJ-1 mRNA的原位杂交，然后通过免疫荧光检测细胞类型标记。我们发现，DJ-1 mRNA表达在大多数神经元在所有的大脑区域检查。特别是，腹侧中脑中的所有多巴胺神经元表达DJ-1 mRNA。与此相反，脉络丛和室管膜细胞衬脑室是唯一的非神经元区域强烈表达DJ-1 mRNA。星形胶质细胞中未检测到DJ-1 mRNA。DJ-1 mRNA在所有黑质多巴胺神经元中表达，但在星形胶质细胞中不表达，这一事实表明其潜在的神经保护作用可能是细胞自主的。此外，DJ-1的表达并不局限于黑质多巴胺神经元，这表明PD连锁突变体DJ-1可能与其他诱发因素相互作用，导致帕金森病中相对选择性的多巴胺神经元变性。
Mutations in DJ-1 cause familial Parkinson's disease (PD). The expression pattern of DJ-1 in the brain remains controversial. In the present study, we used DJ-1 deficient mice as negative controls and examined DJ-1 mRNA expression in mouse brains. In sequential double labeling on the same sections, in situ hybridization of DJ-1 mRNA was followed by immunofluorescence detection of cell type markers. We found that DJ-1 mRNA was expressed in the majority of neurons in all brain areas examined. In particular, all dopamine neurons in the ventral midbrain expressed DJ-1 mRNA. In contrast, the choroid plexus and ependymal cells lining the ventricles were the only non-neuronal regions strongly expressing DJ-1 mRNA. DJ-1 mRNA was not detected in astrocytes. The fact that DJ-1 mRNA is expressed in all nigra dopamine neurons but not in astrocytes suggests that its potential neuroprotective role could be cell-autonomous. Moreover, that DJ-1 expression is not restricted to substantia nigra dopamine neurons suggests that PD-linked mutant DJ-1 may interact with other predisposing factors to cause the relatively selective dopamine neuron degeneration in Parkinson's disease.