Expression of interleukin-34 and colony stimulating factor-1 in the stimulated periodontal ligament cells with tumor necrosis factor-α
Expression of interleukin-34 and colony stimulating factor-1 in the stimulated periodontal ligament cells with tumor necrosis factor-α
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肿瘤坏死因子-α刺激牙周膜细胞中白介素34和集落刺激因子1的表达
DOI：
10.1007/s00795-014-0094-8
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发表时间：
2014
期刊：
影响因子：
1.8
通讯作者：
Nakasho K.
中科院分区：
文献类型：
--
作者：
Kawabe M;Ohyama H;Kato-Kogoe N;Yamada N;Yamanegi K;Nishiura H;Hirano H;Kishimoto H;Nakasho K.
肿瘤坏死因子-α（TNF-α）在破骨细胞生成中直接或间接起着重要作用。然而，TNF-α对集落刺激因子-1受体（CSF-1 R）介导的通过牙周膜（PDL）细胞实现的破骨细胞生成的间接作用尚未完全了解。我们在此研究了在同时存在核因子κ B受体活化剂配体的情况下，用生理高浓度（10 ng/mL）重组TNF-α刺激的人PDL细胞中的5倍上清液诱导破骨细胞分化和成熟的效力。通过用兔IgG中和白细胞介素-34（IL-34）或CSF-1，多核抗酒石酸酸性磷酸酶阳性细胞（但单核细胞除外）的数量减少约50%。使用实时聚合酶链反应在刺激的PDL细胞中测量了少量和大量的IL 34和CSF 1转录本。免疫组化染色和Western blotting检测到IL 34和CSF 1转录产物的表达。此外，使用酶联免疫吸附测定法在刺激的PDL细胞的上清液中测量到0.13 ng/mL的IL-34和5.0 ng/mL的CSF-1。TNF-α刺激的PDL细胞产生的IL-34与CSF-1在CSF-1 R介导的破骨细胞成熟过程中具有协同作用。
Tumor necrosis factor-α (TNF-α) directly and indirectly plays a crucial role in osteoclastogenesis. However, the indirect effects of TNF-α on colony-stimulating factor-1 receptor (CSF-1R)-mediated osteoclastogenesis achieved via periodontal ligament (PDL) cells are not fully understood. We herein examined the potency of osteoclast differentiation and maturation induced by fivefold supernatants in the stimulated human PDL cells with a physiologically high concentration (10 ng/mL) of recombinant TNF-α to human peripheral blood monocytes/macrophages in the simultaneous presence of the receptor activator of nuclear factor kappa-B ligand. The number of tartrate-resistant acid phosphatase-positive cells with multiple nuclei, but not those with a single nucleus, was decreased by approximately 50 % by neutralization with rabbit IgG against either interleukin-34 (IL-34) or CSF-1. Small and large amounts ofIL34andCSF1transcripts were measured in the stimulated PDL cells using real-time polymerase chain reaction. The corresponding amounts of proteins toIL34andCSF1transcripts were observed in the stimulated PDL cells on immunohistochemical staining or Western blotting. Moreover, 0.13 ng/mL of IL-34 and 5.0 ng/mL of CSF-1 were measured in the supernatants of the stimulated PDL cells using an enzyme-linked immunosorbent assay. IL-34 derived from the stimulated PDL cells with TNF-α appeared to synergistically function with CSF-1 in the CSF-1R-mediated maturation of osteoclastogenesis.