The Expression of Osteopontin and Wnt5a in Articular Cartilage of Patients with Knee Osteoarthritis and Its Correlation with Disease Severity.
The Expression of Osteopontin and Wnt5a in Articular Cartilage of Patients with Knee Osteoarthritis and Its Correlation with Disease Severity.
复制标题
膝骨关节炎患者关节软骨中骨桥蛋白、Wnt5a的表达及其与疾病严重程度的相关性
DOI：
10.1155/2016/9561058
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发表时间：
2016
影响因子：
--
通讯作者：
Lei G
中科院分区：
文献类型：
--
作者：
Li Y;Xiao W;Sun M;Deng Z;Zeng C;Li H;Yang T;Li L;Luo W;Lei G
目标.本研究旨在探讨骨桥蛋白（OPN）和Wnt 5a在骨关节炎（OA）发病过程中的作用。方法.选取膝关节骨性关节炎患者和正常对照者的软骨组织50例，根据Mankin改良分级系统分为重度、中度、轻度和正常4组。采用免疫组化和实时荧光定量PCR方法检测骨桥蛋白（OPN）和Wnt 5a在关节软骨中的表达。并探讨骨桥蛋白（OPN）和Wnt 5a表达与OA严重程度的关系。结果OPN和Wnt 5a在四组组织中均能表达。各组间OPN表达水平比较差异有统计学意义（P < 0.01）。Wnt 5a表达强度除轻度组与正常组间差异无统计学意义（P> 0.05）外，其余各组间差异均有统计学意义（P <0.05）。Mankin评分与各组OPN表达（r =-0.847，P < 0.01）和Wnt 5a表达（r =-0.843，P < 0.01）相关。骨桥蛋白与Wnt 5a表达呈正相关（r = 0.769，P < 0.01）。结论在关节软骨中，OPN和Wnt 5a的表达与膝OA关节的进行性损害呈正相关。Wnt 5a与OPN的相关性可能在膝关节OA的发生、发展中起重要作用。
Objectives. This study is undertaken to investigate the relation between osteopontin (OPN) and Wnt5a expression in the progression and pathogenesis of osteoarthritis (OA). Methods. 50 cartilage tissues from knee OA patients and normal controls were divided into four groups of severe, moderate, minor, and normal lesions based on the modified grading system of Mankin. Immunohistochemistry and real-time PCR were utilized to analyze the OPN and Wnt5a expression in articular cartilage. Besides, the relations between OPN and Wnt5a expression and the severity of OA were explored. Results. OPN and Wnt5a could be identified in four groups' tissues. Amongst the groups, the intercomparisons of OPN expression levels showed statistical differences (P < 0.01). Besides, the intercomparisons of Wnt5a expression degrees showed statistical differences (P < 0.05), except that between the minor and normal groups (P > 0.05). The scores of Mankin were demonstrated to relate to OPN expression (r = −0.847, P < 0.01) and Wnt5a expression in every group (r = −0.843, P < 0.01). Also, a positive correlation can be observed between the OPN and Wnt5a expression (r = 0.769, P < 0.01). Conclusion. In articular cartilage, the expressions of OPN and Wnt5a are positively related to progressive damage of knee OA joint. The correlation between Wnt5a and OPN might be important to the progression and pathogenesis of knee OA.