Propionate poses antivirulence activity against Botrytis cinerea via regulating its metabolism, infection cushion development and overall pathogenic factors.
Propionate poses antivirulence activity against Botrytis cinerea via regulating its metabolism, infection cushion development and overall pathogenic factors.
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DOI：
10.1016/j.foodchem.2023.135443
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发表时间：
2023-01
期刊：
影响因子：
8.8
通讯作者：
Chuanxi Zhu;Yan Tang;D. Ren;Weiheng Ren;Yongjun Xue;A. Suthaparan;Jufen Li;Yiwen Wang;Ling Xu;Pinkuan Zhu
中科院分区：
文献类型：
--
作者：
Chuanxi Zhu;Yan Tang;D. Ren;Weiheng Ren;Yongjun Xue;A. Suthaparan;Jufen Li;Yiwen Wang;Ling Xu;Pinkuan Zhu
灰霉病是一种毁灭性的病原体，如果管理不当，会导致水果和蔬菜灰霉病。尽管其机制尚不清楚，但我们之前曾透露，安全食品添加剂丙酸钙（CP）可以抑制葡萄灰霉病的发展。目前的研究报告称，亚致死剂量的 CP（0.2% w/v）可以通过对 B 的全基因组转录本进行大量重编程来实现生长。灰霉病。 CP处理后，与真菌甲基柠檬酸循环（负责丙酸分解代谢）相关的基因上调。同时，CP治疗广泛下调毒力因子的转录水平。多个转录组的进一步比较分析证实，CP处理在很大程度上抑制了与感染垫发育和功能相关的基因的表达。总的来说，这些研究结果表明，CP 不仅可以减少真菌生长，还可以消除真菌毒力因子。因此，CP 在控制果树灰霉病方面具有巨大潜力。
Botrytis cinereais a devastating pathogen causing gray mold in fruits and vegetables if not properly managed. Although the mechanisms remain unclear, we previously revealed that the safe food additive calcium propionate (CP) could suppress gray mold development on grapes. The present study reports that sub-lethal dose of CP (0.2 % w/v) could allow growth with substantial reprograming the genome-wide transcripts ofB. cinerea. Upon CP treatment, the genes related to fungal methylcitrate cycle (responsible for catabolizing propionate) were upregulated. Meanwhile, CP treatment broadly downregulated the transcript levels of the virulence factors. Further comparative analysis of multiple transcriptomes confirmed that the CP treatment largely suppressed the expression of genes related to development and function of infection cushion. Collectively, these findings indicate that CP can not only reduce fungal growth, but also abrogate fungal virulence factors. Thus, CP has significant potential for the control of gray mold in fruit crops.