Spotting effect in microarray experiments
Spotting effect in microarray experiments
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DOI：
10.1186/1471-2105-5-63
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发表时间：
2004-05-19
影响因子：
3
通讯作者：
Hilson, P
中科院分区：
文献类型：
--
作者：
Mary-Huard, T;Daudin, JJ;Hilson, P
Background: Microarray data must be normalized because they suffer from multiple biases. We have identified a source of spatial experimental variability that significantly affects data obtained with Cy3/Cy5 spotted glass arrays. It yields a periodic pattern altering both signal (Cy3/Cy5 ratio) and intensity across the array.Results: Using the variogram, a geostatistical tool, we characterized the observed variability, called here the spotting effect because it most probably arises during steps in the array printing procedure.Conclusions: The spotting effect is not appropriately corrected by current normalization methods, even by those addressing spatial variability. Importantly, the spotting effect may alter differential and clustering analysis.
