The mechanism of enzyme decline in the red blood cell during the "in vivo" aging process.
The mechanism of enzyme decline in the red blood cell during the "in vivo" aging process.
复制标题
“体内”衰老过程中红细胞酶下降的机制。
DOI：
10.1007/978-1-4684-5985-2_10
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发表时间：
1991
影响因子：
--
通讯作者：
Seaman,C
中科院分区：
文献类型：
--
作者：
Piomelli,S;Seaman,C
虽然人们认识到人类红细胞（RBC）具有大约120天的有限寿命，但控制其最终从循环中去除的机制在很大程度上仍然未知。通过研究年龄逐渐增加的细胞群，可以更好地了解RBC衰老的过程。为此，我们的实验室利用浮力密度梯度将RBC分离成比重逐渐增加的组（1，2）。该技术已通过14 C-甘氨酸群标记的RBC实验得到验证，该实验证明标记随细胞年龄从顶部到底部进行性移动（1），以及RBC存活的体内实验，该实验证明最轻的RBC存活时间最长，最重的RBC存活时间最短（3）。在这些数据的基础上，设计了一个数学模型来解释在体内存活方面，观察到的重力逐渐增加的RBC参数的变化（4）。我们实验室的研究表明，从循环中去除衰老RBC的机制之一是代谢活动的逐渐下降（5）。
While it is recognized that the human red blood cell (RBC) has a finite life-span of approximately 120 days, the mechanism that controls its ultimate removal from the circulation remains largely unknown. A better understanding of the process of RBC senescence can be obtained by the study of cohorts of cells of progressively increasing age. Our laboratory has utilized for this purpose separation of RBC into groups of progressively increasing specific gravity utilizing buoyant density gradients (1,2). This technique has been validated by experiments with14C-glycine cohort-labelled RBC that have demonstrated a progressive movement of the label from the top to the bottom with cell age (1) as well as byin vivoexperiments of RBC survival that have demonstrated that the lighest RBC have the longest survival and the heaviest ones, the shortest (3). On the basis of these data a mathematical model has been designed to interpret, in terms ofin vivosurvival, the changes observed in parameters of RBC of progressively increasing gravity (4). Studies from our laboratory suggested that one of the mechanisms for the removal from the circulation of senescent RBC is a progressive decline of metabolic activities (5).