Ultrastructural and genomic characterization of a second banchine polydnavirus confirms the existence of shared features within this ichnovirus lineage
Ultrastructural and genomic characterization of a second banchine polydnavirus confirms the existence of shared features within this ichnovirus lineage
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DOI：
10.1099/vir.0.052506-0
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发表时间：
2013-08-01
影响因子：
3.8
通讯作者：
Cusson, Michel
中科院分区：
文献类型：
--
作者：
Djoumad, Abdelmadjid;Stoltz, Don;Cusson, Michel
多脱氧核糖核酸病毒（Polydnaviruses，PDVs）是由寄生蜂携带并在寄生过程中传播给毛虫宿主的一种共生病毒。虽然它们有几个共同的特征，包括分段的dsDNA基因组，复制仅限于黄蜂宿主的独特生命周期，以及对毛毛虫宿主的免疫抑制/发育作用，但姬蜂和茧蜂携带的PDV（分别称为ichnoviruses和bracoviruses）具有不同的进化起源。此外，ichnoviruses（IV）形成两个不同的谱系，在属于Campopleginae和Banchinae亚科的黄蜂中发现的病毒实体显示出明显不同的病毒粒子形态和基因组特征。然而，目前对banchine IV的描述是基于对单一物种的表征，即烟裂谷盗IV（Glypta fumiferidae IV，GfIV）。在这里，我们提供了第二个banchine IV分离自黄蜂Apophua simpleses的超微结构和基因组分析，我们表明，这种病毒与GfIV有许多共同的特征，包括多核衣壳病毒粒子，聚合基因组大小类似于300 kb，基因组片段
Polydnaviruses (PDVs) are symbiotic viruses carried by endoparasitic wasps and transmitted to caterpillar hosts during parasitization. Although they share several features, including a segmented dsDNA genome, a unique life cycle where replication is restricted to the wasp host, and immunodepressive/developmental effects on-the caterpillar host, PDVs carried by ichneumonid and braconid wasps (referred to as ichnoviruses and bracoviruses, respectively) have different evolutionary origins. In addition, ichnoviruses (IVs) form two distinct lineages, with viral entities found in wasps belonging to the subfamilies Campopleginae and Banchinae displaying strikingly different virion morphologies and genomic features. However, the current description for banchine IVs is based on the characterization of a single species, namely that of the Glypta fumiferanae IV (GfIV). Here we provide an ultrastructural and genomic analysis of a second banchine IV isolated from the wasp Apophua simplicipes, and we show that this virus shares many features with GfIV, including a multi-nucleocapsid virion, an aggregate genome size of similar to 300 kb, genome segments