Enhanced Metabolism Evolved High-Level Resistance to Fenoxaprop-P-Ethyl in Alopecurus japonicus
Enhanced Metabolism Evolved High-Level Resistance to Fenoxaprop-P-Ethyl in Alopecurus japonicus
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代谢增强导致看婆对精恶唑禾草灵产生高水平耐药性
DOI：
10.3390/agronomy12092172
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发表时间：
2022-09
期刊：
影响因子：
--
通讯作者：
Lang Pan
中科院分区：
文献类型：
--
作者：
Zongfang Li;Haozhe Liu;Junzhi Wang;Wen Chen;Lianyang Bai;Lang Pan
看麦娘（Alopecurus）是我国重要的杂草，对乙酰辅酶A羧化酶抑制剂（ACCase）产生了抗药性。助理靶位点耐药（TSR）的原因已明确，而非靶位点耐药（NTSR）的原因尚不清楚。剂量反应试验表明，A.抗性群体（R）对4种ACCase抑制型除草剂精恶唑禾草灵、稀禾定、烯草酮和唑啉草均表现抗性。与敏感A.因此（S），使用测序在R群体中未鉴定出ACCase中TSR的已知耐药突变。为了研究NTSR机制，使用高效液相色谱（HPLC）分析在R种群中检测到精恶唑禾草灵的代谢增加。值得注意的是，耐药性不能被P450和GST抑制剂逆转。通过RNA-seq进一步探索耐药机制，选择了8个候选重叠群（4个糖基转移酶（GT）和4个ATP结合盒（ABC）转运蛋白），并使用RT-qPCR验证其表达模式。三个GT和三个ABC转运蛋白重叠群在R群体中组成性上调。简而言之，在R群体中高度表达的六个重叠群引起精恶唑禾草灵代谢增强，似乎参与精恶唑禾草灵抗性。
Alopecurus japonicus has been a serious weed across China and developed resistance to the acetyl-CoA carboxylase (ACCase)-inhibiting herbicide. The A. japonicus ACCase gene mutations accounting for target-site resistance (TSR) have been clarified, while non-target-site resistance (NTSR) is not distinct. Here, dose–response testing indicated that an A. japonicus population (R) was resistant to four ACCase-inhibiting herbicides fenoxaprop-P-ethyl, sethoxydim, clethodim, and pinoxaden. Compared with herbicide-susceptible A. japonicus (S), no known resistant mutations for TSR in ACCase were identified in the R population using sequencing. To investigate the NTSR mechanism, increased metabolism of fenoxaprop-P-ethyl was detected in the R population using high-performance liquid chromatography (HPLC) analysis. Notably, resistance cannot be reversed by P450 and GST inhibitors. RNA-seq was performed to further explore the resistance mechanisms, and eight candidate contigs (four glycosyltransferases (GT) and four ATP-binding cassette (ABC) transporters) were chosen and their expression patterns were validated using RT-qPCR. Three GT and three ABC transporter contigs were constitutively upregulated in the R population. In short, six contigs expressed highly in the R population causing enhanced fenoxaprop-P-ethyl metabolism appear to be involved in fenoxaprop-P-ethyl resistance.
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DOI：
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发表时间：
2016
期刊：
--
影响因子：
--
作者：
M. Grabherr;B. Haas;M. Yassour;J. Levin;Dawn A Thompson;I. Amit;Xian Adiconis;Lin Fan;R. Raychowdhu
通讯作者：
M. Grabherr;B. Haas;M. Yassour;J. Levin;Dawn A Thompson;I. Amit;Xian Adiconis;Lin Fan;R. Raychowdhu
影响因子：
4.7
作者：
Hongle Xu;Xu-dong Zhu;Hongchun Wang;Jun Yu Li;Liyao Dong
通讯作者：
Hongle Xu;Xu-dong Zhu;Hongchun Wang;Jun Yu Li;Liyao Dong
醛酮还原酶代谢草甘膦并赋予稗草抗性
影响因子：
7.4
作者：
Pan, Lang;Yu, Qin;Powles, Stephen
通讯作者：
Powles, Stephen
DOI：
10.1111/pce.12017
发表时间：
2013-04
期刊：
Plant, cell & environment
影响因子：
--
作者：
Q. Yu;Heping Han;Greg Cawthray;Shaofang Wang;S. Powles
通讯作者：
Q. Yu;Heping Han;Greg Cawthray;Shaofang Wang;S. Powles
针对癌症转录组的下一代测序可增强序列变体和新型融合转录本的检测。
影响因子：
12.3
作者：
Levin JZ;Berger MF;Adiconis X;Rogov P;Melnikov A;Fennell T;Nusbaum C;Garraway LA;Gnirke A
通讯作者：
Gnirke A