A His-tagged Melanocarpus albomyces laccase and its electrochemistry upon immobilisation on NTA-modified electrodes and in conducting polymer films.
A His-tagged Melanocarpus albomyces laccase and its electrochemistry upon immobilisation on NTA-modified electrodes and in conducting polymer films.
复制标题
His 标记的黑果酵母漆酶及其固定在 NTA 修饰电极和导电聚合物薄膜上的电化学。
DOI：
10.1002/cphc.201300340
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
P. Bartlett
中科院分区：
文献类型：
--
作者：
M. Sosna;Harry Boer;P. Bartlett
本文介绍了白果黑果组氨酸标记漆酶的构建、固定化和电化学。介绍了一种利用重氮接枝和固相化学的方法，用氮三乙酸(NTA)对玻碳电极进行功能化处理的简便方法。NTA修饰电极与漆酶结合，漆酶在可溶性氧化还原介体的存在下，在中性pH条件下还原氧气。漆酶修饰电极也是通过在聚苯胺膜/聚乙烯基磺酸盐膜中固定化酶来制备的。该聚合物被发现有效地保留了酶，并在电极和酶活性中心之间提供了直接的电接触。循环伏安法表明，直接电子转移到酶依赖于聚合物膜的氧化还原状态。
The article describes the construction, immobilisation and electrochemistry of histidine tagged laccase from Melanocarpus albomyces. A facile method of functionalisation of glassy carbon electrodes with nitrilotriacetic acid (NTA) using diazonium grafting and solid state chemistry is described. NTA-modified electrodes are shown to bind laccase which reduces oxygen at neutral pH in the presence of soluble redox mediator. Laccase-modified electrodes are also prepared by enzyme immobilisation within poly(aniline)/poly(vinylsulfonate) films. The polymer is found to efficiently retain the enzyme as well as provide direct electrical contact between the electrode and the enzyme active centre. Cyclic voltammetry reveals the direct electron transfer to the enzyme is dependent on the redox state of the polymer film.