Acetylcholinesterase in insecticide resistant Culex tritaeniorhynchus: Characteristics accompanying insensitivity to inhibitors
Acetylcholinesterase in insecticide resistant Culex tritaeniorhynchus: Characteristics accompanying insensitivity to inhibitors
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DOI：
10.1303/aez.32.37
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发表时间：
1997-02-01
影响因子：
1.3
通讯作者：
Kono, Y
中科院分区：
文献类型：
--
作者：
Mamiya, A;Ishikawa, Y;Kono, Y
三带喙库蚊富山株的乙酰胆碱酯酶(AChE)对各种有机磷杀虫剂和氨基甲酸酯类的敏感性极低。对富山氏吸虫AChE的几个特性进行了检测，并与台湾地区的OP敏感株进行了比较。富山的伤痛与台湾有许多不同之处。没有明确的最适pH和底物浓度(台湾AChE同时存在最适pH和底物浓度)。对ACh的亲和力很高(K-m值是台湾的0.65倍)，但对底物乙酰硫胆碱和丙酰硫胆碱的亲和力显著下降。有机磷农药的I-50值极高(是台湾的66.1-7,079倍)，氨基甲酸酯的I-50值也高于台湾(20.4-112倍)。这些结果表明，富山的AChE结构已经改变为ACh专一形式，与OP化合物的亲和力明显降低。
The acetylcholinesterase (AChE) of the Toyama strain (Toyama) of Culex tritaeniorhynchus showed extremely low sensitivity to various kinds of organophosphorus insecticides (OPs) and carbamates. Several characteristics of the AChE of Toyama have been tested and compared with those of the OP susceptible strain (Taiwan). The AChE of Toyama is different from that of Taiwan in many respects. It showed no clear optimum pH and substrate concentration (AChE of Taiwan had both the optimum pH and substrate concentration). Affinity for ACh was high (K-m value for ACh is 0.65 times the value of Taiwan), but affinity for the substrate declined significantly for acetylthiocholine and propionylthiocholine. I-50 values for OPs were extremely high (66.1-7,079 times the value for Taiwan), and those for carbamates were also higher than those for Taiwan (20.4-112 times). These results suggest that the structure of the AChE of Toyama has changed to an ACh specific form which has a remarkably lower affinity to the OP compounds.