MONOCLONAL-ANTIBODIES TO RESPIRATORY SYNCYTIAL VIRUS PROTEINS - IDENTIFICATION OF THE FUSION PROTEIN
MONOCLONAL-ANTIBODIES TO RESPIRATORY SYNCYTIAL VIRUS PROTEINS - IDENTIFICATION OF THE FUSION PROTEIN
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DOI：
10.1128/jvi.47.1.171-177.1983
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发表时间：
1983-01-01
影响因子：
5.4
通讯作者：
HRUSKA, J
中科院分区：
文献类型：
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作者：
WALSH, EE;HRUSKA, J
制备了6株针对呼吸道合胞病毒蛋白的小鼠单抗。免疫沉淀法和间接免疫荧光法对每种方法进行鉴定。一个针对核衣壳蛋白，NP 44，2针对37,000道尔顿蛋白，2针对主要包膜糖蛋白GP 90，1针对70,000道尔顿包膜蛋白VP 70。感染的人喉癌Hep-2细胞的间接免疫荧光染色图谱表明，GP 90和VP 70是细胞表面表达的病毒蛋白。检测到NP 44和37,000道尔顿蛋白为胞质内包涵体。其中一种抗GP 90抗体仅在补体存在的情况下中和病毒，但不抑制细胞与细胞的融合。抗VP70抗体中和无补体的病毒，并抑制先前感染的Hep-2细胞的细胞-细胞融合，从而确定VP70为融合蛋白。
Six mouse monoclonal antibodies directed against respiratory syncytial virus proteins were produced. Each was characterized by immunoprecipitation and indirect immunofluorescence. One was directed against the nucleocapsid protein, NP 44, 2 were directed against a 37,000-dalton protein, 2 were directed against the major envelope glycoprotein, GP 90, and 1 was directed against the 70,000-dalton envelope protein, VP 70. Indirect immunofluorescence stain patterns of infected human laryngeal carcinoma HEp-2 cells defined GP 90 and VP 70 as viral proteins expressed on the cell surface. NP 44 and the 37,000-dalton protein were detected as intracytoplasmic inclusions. One of the anti-GP 90 antibodies neutralized virus only in the presence of complement but did not inhibit cell-cell fusion. The anti-VP 70 antibody neutralized virus without complement and inhibited cell-cell fusion of previously infected HEp-2 cell, thus identifying VP 70 as the fusion protein.