CIRCUIT ANALYSIS OF THE OSCILLATORY STATE IN GLYCOLYSIS
CIRCUIT ANALYSIS OF THE OSCILLATORY STATE IN GLYCOLYSIS
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DOI：
10.1016/0303-2647(89)90064-6
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发表时间：
1989-01-01
期刊：
影响因子：
1.6
通讯作者：
BUSSE, HG
中科院分区：
文献类型：
--
作者：
BOITEUX, A;BUSSE, HG
用电子电路研究中已知的方法分析了酵母提取物中糖酵解的振荡状态。反应的时间过程用最小二乘法从实验确定的大多数代谢物的浓度集合中计算出来。糖酵解反应网络的动力学可以用流量与驱动力（相应电子电路中的电流与电压）的关系来表示。动力学分析得出结论，糖酵解在振荡状态下以脉冲方式开启和关闭。所产生的脉冲流量不仅可以用糖酵解的最终产物，如二氧化碳或乙醇来测量，而且可以很容易地用单酶步骤的反应速率图来证明，甚至在糖酵解序列的初始阶段。一种广泛应用于电子电路的分析方法也被证明可用于研究复杂酶网络的动力学。
The oscillatory state of glycolysis in yeast extracts has been analysed by methods known from electronic circuit studies. The time course of the reactions are calculated by the method of least squares from experimentally determined sets of the concentrations of most of the metabolites. The dynamics of the glycolytic network of reactions can then be represented in terms of flow versus driving force (current versus voltage in the corresponding electronic circuit). The analysis of the dynamics leads to the conclusion that glycolysis is switched on and off in a pulsed manner during the oscillatory state. The resulting pulsed flow cannot only be measured with glycolytic end products, like carbon dioxide or ethanol, but can also readily be demonstrated by diagrams of reaction rates of single enzymic steps even in the initial stages of the glycolytic sequence. An analytic method widely applied to electronic circuits also proved to be useful in the study of the dynamics of a complex enzymic network.