RATE OF DEPURINATION OF NATIVE DEOXYRIBONUCLEIC ACID
RATE OF DEPURINATION OF NATIVE DEOXYRIBONUCLEIC ACID
复制标题
DOI：
10.1021/bi00769a018
复制
发表时间：
1972-01-01
期刊：
影响因子：
2.9
通讯作者：
NYBERG, B
中科院分区：
文献类型：
--
作者：
LINDAHL, T;NYBERG, B
材料和方法 DNA 制剂。需要嘌呤的枯草芽孢杆菌突变体（菌株 168 purB 6）获自 A. Adams 博士。细菌生长在O’Sullivan and Sueoka (1967)的C+感受态培养基中，添加5 X 10”·m [8-14C]腺嘌呤(20 mCi/mmole)。细菌在对数生长期收获，用含10~4 m的0.15 m NaCl-0.1 m EDTA (pH 7.0)洗涤一次。腺嘌呤， 悬浮在相同的溶剂中，并用1 mg/ml的3610生物化学，卷。 11、没有。 1972 年 19 日
Materials and MethodsDNA Preparations. A purine-requiring mutant of Bacillus subtilis (strain 168 purB 6) was obtained from Dr. A. Adams. The bacteria were grown in the competence medium C+ of O’Sullivan andSueoka (1967), supplemented with 5 X 10 “·’m [8-14C] adenine (20 mCi/mmole). The bacteria were har-vested in the late logarithmic growth phase, washed once with 0.15 m NaCl-0.1 m EDTA (pH 7.0) containing 10~ 4 m adenine, suspended in the same solvent, and treated with 1 mg/ml of3610 biochemistry, vol. 11, no. 19, 1972