LOCALIZATION OF NADPH-DIAPHORASE-CONTAINING NEURONS IN SENSORY GANGLIA OF THE RAT
LOCALIZATION OF NADPH-DIAPHORASE-CONTAINING NEURONS IN SENSORY GANGLIA OF THE RAT
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DOI：
10.1002/cne.903060303
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发表时间：
1991-04-15
影响因子：
2.5
通讯作者：
KIMURA, H
中科院分区：
文献类型：
--
作者：
AIMI, Y;FUJIMURA, M;KIMURA, H
还原型烟酰胺腺嘌呤二核苷酸磷酸（NADPH）-心肌黄酶活性的存在下进行了组织化学研究在感觉神经节的大鼠。 脊上神经节内只有少数细胞呈NADPH黄递酶阳性，而结状神经节内有大量阳性神经元。 在背根神经节，阳性细胞的分布与脊髓水平呈特殊的关系。 极少数群体（小于总神经节细胞的2%）在第一颈椎（C1）至第四胸椎（T4）和第二腰椎（L2）至整个骶骨节段的神经节中表现出阳性反应。 然而，在中至下胸水平（从T5到L1），观察到丰富的心肌黄酶阳性细胞。 从这些阳性神经元中可以追踪到染色强烈的神经纤维。 例如，在下胸段，在交通支中观察到密集的阳性纤维。 逆行追踪研究表明，含心肌黄酶的神经元在下胸部水平的项目至少部分胃壁和腹腔神经节。 这些结果提示胸腰段心肌黄酶阳性神经节细胞可能携带自主内脏传入信息。 NADPH-心肌黄酶组织化学和肽免疫组织化学双重染色显示，在许多内脏传入神经元中，NADPH-心肌黄酶与降钙素基因相关肽和P物质共定位。
The presence of reduced nicotinamide adenine dinucleotide phosphate (NADPH)-diaphorase activity was studied histochemically in the sensory ganglia of the rat. Supraspinally, the trigeminal ganglion possessed only a few cells positively stained for NADPH-diaphorase, while a large number of positive neurons was found in the nodose ganglion. In the dorsal root ganglia, the distribution of positive cells showed a peculiar pattern in relation to spinal levels. Very minor populations (less than 2% of the total ganglionic cells) exhibited positive reaction in ganglia at levels ranging from the first cervical (C1) to fourth thoracic (T4) and from the second lumber (L2) through the entire sacral levels. In the middle to lower thoracic levels (from T5 to L1), however, abundant diaphorase-positive cells were observed. From these positive neurons it was possible to trace intensely stained nerve fibers. In the lower thoracic level, for example, dense positive fibers were seen in the ramus communicans. Retrograde tracing studies revealed that diaphorase-containing neurons in the lower thoracic level project at least partly to the gastric wall and the celiac ganglion. These results indicate that the diaphorase-positive ganglionic neurons in the thoracicolumbar levels may carry autonomic visceral afferent information. Double staining with NADPH-diaphorase histochemistry and peptide immunohistochemistry revealed that NADPH-diaphorase colocalizes with calcitonin gene-related peptide and substance P in many of these visceral afferent neurons.