Involvement of the Arabidopsis SWI2/SNF2 chromatin remodeling gene family in DNA damage response and recombination
Involvement of the Arabidopsis SWI2/SNF2 chromatin remodeling gene family in DNA damage response and recombination
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DOI：
10.1534/genetics.105.051664
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发表时间：
2006-06-01
期刊：
影响因子：
3.3
通讯作者：
Levy, Avraham A.
中科院分区：
文献类型：
--
作者：
Shaked, Hezi;Avivi-Ragolsky, Naomi;Levy, Avraham A.
与其他真核生物一样，植物的基因组被组织成染色质，染色质是一种紧凑的结构，它降低了DNA对转录、复制和DNA重组和修复等机制的可及性。含有染色质重塑蛋白Swi 2/Snf 2家族的特征性ATP酶/解旋酶基序的植物基因已被深入研究，但它们在同源重组或DNA修复中的作用受到的关注有限。我们已经搜索了酵母RAD 54基因的同源物，其在重组和修复以及染色质重塑中的作用是公认的。鉴定了40个拟南芥SWI 2/SNF 2基因，并分析了选定的14个基因组的功能。突变分析和/或RNAi介导的沉默显示，测试的14个基因中有11个在响应DNA损伤中发挥作用。14个基因中有2个参与了反向重复序列之间的同源重组。推测的RAD 54的直系同源物和ERCC 6/RAD 26的近同源物参与DNA损伤反应，表明跨界的功能保守性。此外，已知在发育中起作用的基因，如PICKLE/GYMNOS和PIE 1，或沉默中的基因，如DDM 1，也参与了DNA损伤反应。ddm 1和met 1突变体的比较表明，DNA损伤反应主要受染色质结构的影响，胞嘧啶甲基化不太重要。这些结果强调了SWI 2/SNF 2家族的广泛参与，从而染色质重塑，在基因组的维护和表观遗传和遗传过程之间的联系。
The genome of plants, like that of other eukaryotes, is organized into chromatin, a compact structure that reduces the accessibility of DNA to machineries such as transcription, replication, and DNA recombination and repair. Plant genes, which contain the characteristic ATPase/helicase motifs of the chromatin remodeling Swi2/Snf2 family of proteins, have been thoroughly studied, but their role in homologous recombination or DNA repair has received limited attention. We have searched for homologs of the yeast RAD54 gene, whose role in recombination and repair and in chromatin remodeling is well established. Forty Arabidopsis SWI2/SNF2 genes were identified and the function of a selected group of 14 was analyzed. Mutant analysis and/or RNAi-mediated silencing showed that 11 of the 14 genes tested played a role in response to DNA damage. Two of the 14 genes were involved in homologous recombination between inverted repeats. The putative ortholog of RAD54 and close homologs of ERCC6/ RAD26 were involved in DNA damage response, Suggesting functional conservation across kingdoms. In addition, genes known for their role in development, such as PICKLE/GYMNOS and PIE1, or in silencing, Such as DDM1, turned Out to also be involved in DNA damage response. A comparison of ddm1 and met1 Mutants suggests that DNA damage response is affected essentially by chromatin structure and that cytosine methylation is less critical. These results emphasize the broad involvement of the SWI2/SNF2 family, and thus of chromatin remodeling, in genome maintenance and the link between epigenetic and genetic processes.