Response of denitrifying community, denitrification genes and antibiotic resistance genes to oxytetracycline stress in polycaprolactone supported solid-phase denitrification reactor
Response of denitrifying community, denitrification genes and antibiotic resistance genes to oxytetracycline stress in polycaprolactone supported solid-phase denitrification reactor
复制标题
聚己内酯固相反硝化反应器中反硝化群落、反硝化基因和抗生素抗性基因对土霉素胁迫的响应
DOI：
10.1016/j.biortech.2020.123274
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发表时间：
2020-07-01
影响因子：
11.4
通讯作者：
Zhou, Jiaheng
中科院分区：
文献类型：
--
作者：
Feng, Lijuan;Yang, Jingyi;Zhou, Jiaheng
废水中硝酸盐与抗生素共存是一个普遍存在的问题。研究了聚己内酯（PCL）支持的固相反硝化反应器中反硝化菌群、反硝化基因和抗生素抗性基因对土霉素（OTC）胁迫的响应。在3个月的运行过程中，在0、0.05、0.25和1 mg L-1的土霉素胁迫下，SPD系统的硝酸盐还原率均达到99%以上，而当土霉素浓度进一步增加到5 mg L-1时，SPD系统的硝酸盐还原率显著下降约5%。高效反硝化与反硝化菌的多样性密切相关，但当土霉素浓度达到>= 0.25 mg L-1时，反硝化菌的丰度急剧下降，导致反硝化基因narH、narJ、narI nirD、nosZ和norB显著下降。四环素耐药基因是不同OTC胁迫促进ARG的主要类型，主要与tet 36、tetG、tetA、tetM和tetC的增加有关。
The coexistence of nitrate and antibiotics in wastewater is a common problem. The study aimed to explore the response of denitrifying community, denitrification genes and antibiotic resistance genes (ARGs) to oxytetracycline (OTC) stress in polycaprolactone (PCL) supported solid-phase denitrification (SPD) reactors. Complete nitrate reduction (greater than99%) was achieved in SPD system with OTC stress of 0, 0.05, 0.25 and 1 mg L-1 during three-month operation, while it significantly declined by about 5% at a further increased OTC level of 5 mg L-1. The efficient denitrification strongly related with a rich diversity of denitrifiers, while the abundances of which dramatically reduced as the OTC concentration reached >= 0.25 mg L-1, which caused significant decline of denitrification genes, especially for narH, narJ, narI nirD, nosZ, and norB. Tetracycline resistance genes were a major type of promoted ARGs by different OTC stress, mainly related with the increase of tet36, tetG, tetA, tetM and tetC.