Effect of winter-flooding on methanogenic archaeal community structure in paddy field under organic farming
Effect of winter-flooding on methanogenic archaeal community structure in paddy field under organic farming
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DOI：
10.1080/00380768.2012.726598
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发表时间：
2012-10
影响因子：
2
通讯作者：
Dongyan Liu;Chika Suekuni;K. Akita;Toyoaki Ito;M. Saito;Takeshi Watanabe;M. Kimura;S. Asakawa
中科院分区：
文献类型：
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作者：
Dongyan Liu;Chika Suekuni;K. Akita;Toyoaki Ito;M. Saito;Takeshi Watanabe;M. Kimura;S. Asakawa
稻田是甲烷的主要排放源。甲烷是稻田有机质厌氧分解的最终产物，由产甲烷古菌在淹水条件下产生。本研究旨在揭示冬季淹水对有机耕作条件下水稻土产甲烷古菌群落结构的影响。从东北大学田间科学中心的实验稻田中收集土壤样品，为期两年。他们在冬季有机农业期间处于洪水条件下，在冬季有机农业期间处于非洪水条件下，在冬季传统农业（非有机农业）期间处于非洪水条件下。聚合酶链反应-变性梯度凝胶电泳（PCR-DGGE）分析的产甲烷古菌16 S rRNA基因显示，DGGE模式几乎是相同的治疗和采样时间无关。每个处理共检测到23条条带，与甲烷微生物目、甲烷微生物目和甲烷细胞目密切相关的序列分别为4、13和6条。实时荧光定量PCR分析结果表明，稻田土壤中甲烷古菌16 S rRNA基因和甲基辅酶M还原酶α亚基基因mcrA的丰度差异不显著。这项研究首次揭示了一个产甲烷古菌群落在安多索尔稻田，并表明该社区没有受到冬季洪水有机耕作。
Paddy field is a major emission source of methane. Methane is the terminal product of anaerobic decomposition of organic matter and generated by methanogenic archaea under flooded conditions in paddy fields. This study aimed to reveal the effect of winter flooding on methanogenic archaeal community structure in paddy fields of Andosols under organic farming. Soil samples were collected from experimental paddy fields in the Field Science Center, Tohoku University, for two years. They were under flooding conditions during winter with organic farming, under non-flooding conditions during winter with organic farming and under non-flooding conditions during winter with conventional farming (non-organic farming). Polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) analysis of methanogenic archaeal 16S rRNA gene revealed that the DGGE patterns were nearly the same irrespective of the treatment and sampling times. Twenty-three bands were observed from each treatment and 4, 13 and 6 sequences were closely related to Methanomicrobiales, Methanosarcinales and Methanocellales, respectively. Real-time quantitative PCR analysis indicated that the abundance of methanogenic archaeal 16S rRNA gene and mcrA gene, encoding α subunit of methyl-coenzyme M reductase, was not significantly different among the paddy fields. This study first revealed a methanogenic archaeal community in an Andosol paddy field and showed that the community was not affected by winter flooding under organic farming.