In vivo MR spectroscopy and MR imaging on non-anaesthetized marine fish:: techniques and first results
In vivo MR spectroscopy and MR imaging on non-anaesthetized marine fish:: techniques and first results
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DOI：
10.1016/s0730-725x(02)00482-4
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发表时间：
2002-02-01
影响因子：
2.5
通讯作者：
Pörtner, HO
中科院分区：
文献类型：
--
作者：
Bock, C;Sartoris, FJ;Pörtner, HO
使用4.7 T水平磁铁对最大体长为40 cm的非麻醉海鱼进行体内磁共振成像（MRI）和光谱学（MRS）实验。在流通式动物室中，可以对固着的底栖动物种类（Eelpout Zoarces viviparus）以及远洋鳕鱼种类（Gadus morhua）进行数天的长期研究。适应海水的探头以及优化的激励和重新聚焦脉冲形状减少了高导电介质的负面影响。在这两个物种中，在 5 分钟内收集了体内 P-31-NMR 谱，具有合理的信噪比（鳕鱼中的 PCr/Pi 比率 > 40）和典型线宽在 20 至最大范围内。 40赫兹。磁共振图像是在没有运动伪影的无柄物种中记录的。在这些鱼中，甚至可以使用流量补偿梯度回波序列来测量不同血管的血流量，并在不同器官中进行局部体内 H-1-NMR 波谱分析。 (C) 2002 Elsevier Science Inc. 保留所有权利。
In vivo Magnetic Resonance Imaging (MRI) and Spectroscopy (MRS) experiments were carried out in non-anaesthetized marine fish with a maximum body length of 40 cm using a 4.7 T horizontal magnet. In flow-through animal chambers long-term investigations could be carried out for several days in a sessile benthic zoarcid species, the eelpout Zoarces viviparus, as well as in a pelagic gadid species, the cod Gadus morhua. Sea water adapted probes and optimised excitation and refocusing pulse shapes reduced the negative effects of the highly conductive medium. In both species, in vivo P-31-NMR spectra were collected within 5 minutes with a reasonable signal to noise ratio (PCr/Pi ratio > 40 in cod) and typical line widths in the range of 20 to max. 40 Hz. Magnetic resonance images were recorded in the sessile species without movement artefacts. In these fish it was even possible to measure the blood flow of different vessels with a flow compensated gradient echo sequence and to carry out localized in vivo H-1-NMR spectroscopy in different organs. (C) 2002 Elsevier Science Inc. All rights reserved.