The identification of an endonuclease that cleaves within an HuR binding site in mRNA.
The identification of an endonuclease that cleaves within an HuR binding site in mRNA.
复制标题
鉴定在 mRNA 的 HuR 结合位点内进行切割的核酸内切酶。
DOI：
10.1093/nar/28.14.2695
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发表时间：
2000
影响因子：
14.9
通讯作者：
Furneaux,HM
中科院分区：
文献类型：
--
作者：
Zhao,Z;Chang,FC;Furneaux,HM
含有富含 U 元素的信使 RNA (mRNA) 是快速衰变的目标。选择性抑制这种衰变会导致稳态水平快速增加。因此，这是基因表达的重要调控步骤。此前，我们发现这些 mRNA 被一种称为 HuR 的特定 mRNA 结合蛋白选择性地稳定。 HuR 的作用机制尚不清楚。据推测，HuR 通过置换或抑制特异性切割或去腺苷酸化这些 mRNA 的因子来稳定 mRNA。在本文中，我们报告了一种新型核酸内切酶的鉴定和表征，该酶可在 p27kip1 mRNA 的 HuR 结合位点内进行切割。这种核酸内切酶的特异性及其受到 HuR 的抑制表明它在基因表达的转录后调控中发挥作用。
Messenger RNAs (mRNAs) that contain U-rich elements are targeted for rapid decay. Selective inhibition of this decay results in a rapid increase in steady state level. Thus, this is an important regulatory step in gene expression. Previously, we have found that these mRNAs are selectively stabilized by a specific mRNA binding protein called HuR. The mechanism of action of HuR is not well understood. It has been postulated that HuR stabilizes mRNA by the displacement or inhibition of factors that specifically cleave or deadenylate these mRNAs. In this paper, we report the identification and characterization of a novel endonuclease that cleaves within an HuR binding site in p27kip1 mRNA. The specificity of this endonuclease and its inhibition by HuR argue for it playing a role in the postranscriptional regulation of gene expression.