Isolation and identification of a sodium channel-inhibiting protein from eggs of black widow spiders
Isolation and identification of a sodium channel-inhibiting protein from eggs of black widow spiders
复制标题
从黑寡妇蜘蛛卵中分离和鉴定钠通道抑制蛋白。
DOI：
10.1016/j.ijbiomac.2014.01.004
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发表时间：
2014-04-01
影响因子：
8.2
通讯作者：
Liang, Songping
中科院分区：
文献类型：
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作者：
Li, Jianjun;Yan, Yizhong;Liang, Songping
黑寡妇蜘蛛(L.tredecimgutatus)的卵含有丰富的生物活性成分，具有很大的研究价值和应用前景。本研究采用凝胶过滤、离子交换层析和反相高效液相色谱相结合的方法，从鸡蛋中分离出一种蛋白毒素，命名为Latroeggoxin-II。电子光谱分析表明，该蛋白的相对分子质量为28.69 kDa，Edman降解结果表明其N端序列为ESIQT STYVP NTPNQKFDYEVGKDY-。这种蛋白在小鼠和美洲对虾体内注射后，可能会使这些动物，特别是美洲对虾出现一系列中毒症状。电生理实验表明，该蛋白能选择性地抑制大鼠背根神经节神经元对河豚毒素敏感的钠通道电流，而对河豚毒素敏感的钠通道电流无明显影响。利用多种蛋白质组学策略，纯化的蛋白质与数据库中现有的蛋白质只有几个相似之处，这表明它是从黑寡妇蜘蛛的卵中分离出来的一种新蛋白质。(C)爱思唯尔出版的2014年。
The eggs of black widow spider (L. tredecimguttatus) have been demonstrated to be rich in biologically active components that exhibit great research value and application foreground. In the present study, a protein toxin, named Latroeggtoxin-II, was isolated from the eggs using the combination of gel filtration, ion exchange chromatography and reversed-phase high performance liquid chromatography. Electraspray mass spectrometric analysis indicated that the molecular weight of the protein was 28.69 kDa, and Edman degradation revealed that its N-terminal sequence was ESIQT STYVP NTPNQKFDYEVGKDY-. After being abdominally injected into mice and P. americana, the protein could make the animals especially P. americana display a series of poisoning symptoms. Electrophysiological experiments demonstrated that the protein could selectively inhibit tetrodotoxin-resistant Na+ channel currents in rat dorsal root ganglion neurons, without significant effect on the tetrodotoxin-sensitive Na+ channel currents. Using multiple proteomic strategies, the purified protein was shown to have only a few similarities to the existing proteins in the databases, suggesting that it was a novel protein isolated from the eggs of black widow spiders. (C) 2014 Published by Elsevier B.V.