Distribution of invariant natural killer T cells and dendritic cells in late pre-term birth without acute chorioamnionitis
Distribution of invariant natural killer T cells and dendritic cells in late pre-term birth without acute chorioamnionitis
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DOI：
10.1111/aji.12658
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发表时间：
2017-06-01
影响因子：
3.6
通讯作者：
Takahashi, Hidemi
中科院分区：
文献类型：
--
作者：
Negishi, Yasuyuki;Shima, Yoshio;Takahashi, Hidemi
问题：急性绒毛膜羊膜炎（aCAM）是早产的重要原因。然而，对于无aCAM的晚期早产的发病机制知之甚少，这是最常见的早产类型。在这里，我们分析了从晚期早产妇女蜕膜中获得的免疫细胞动力学，有和没有aCAM。研究方法：从没有aCAM （PB-n/aCAM）或有aCAM （PB-w/aCAM）的晚期早产分娩妇女中获得蜕膜。流式细胞术分析DEC-205（+）树突状细胞（dc）、巨噬细胞、不变自然杀伤T细胞（iNKT）、NK细胞、CD8(+) T细胞和CD4(+) T细胞的数量。结果：PB-n/aCAM患者蜕膜中iNKT细胞数量明显高于PB-w/aCAM。从携带PB-n/aCAM的女性中获得的DEC-205(+) dc优先诱导iNKT细胞增殖。结论：iNKT细胞在PB-n/aCAM中聚集DEC-205(+) dc，提示iNKT细胞参与了PB-n/aCAM的发病。
Problem: Acute chorioamnionitis (aCAM) is an important cause of pre-term birth. However, little is known about the pathogenesis of late pre-term birth without aCAM that was the most common category of pre-term birth. Here we analyze the kinetics of immune cells obtained from the decidua of women with late pre-term births with and without aCAM.Method of study: Deciduas were obtained from women who underwent labor with late pre-term birth without aCAM (PB-n/aCAM) or with aCAM (PB-w/aCAM). The population of DEC-205(+) dendritic cells (DCs), macrophages, invariant natural killer T (iNKT) cells, NK cells, CD8(+) T cells, and CD4(+) T cells were analyzed by flow cytometry.Results: The number of iNKT cells was higher in the decidua obtained from women with PB-n/aCAM than PB-w/aCAM. DEC-205(+) DCs obtained from women with PB-n/aCAM preferentially induced iNKT cell proliferation.Conclusion: iNKT cell accumulation with DEC-205(+) DCs in PB-n/aCAM suggests that iNKT cells contribute to the onset of PB-n/aCAM.