The WNT inhibitor APCDD1 sustains the expression of β-catenin during the osteogenic differentiation of human dental follicle cells.
The WNT inhibitor APCDD1 sustains the expression of β-catenin during the osteogenic differentiation of human dental follicle cells.
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DOI：
10.1016/j.bbrc.2014.12.107
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发表时间：
2015-02
影响因子：
3.1
通讯作者：
S. Viale-Bouroncle;C. Klingelhöffer;T. Ettl;C. Morsczeck
中科院分区：
文献类型：
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作者：
S. Viale-Bouroncle;C. Klingelhöffer;T. Ettl;C. Morsczeck
在毛囊细胞中，APCDD 1抑制经典的WNT/β-连环蛋白途径，其失活与常染色体显性形式的脱发相关。我们分析了APCDD 1在牙囊细胞（DFC）成骨分化中的作用，并确定了一个新的和令人惊讶的功能。与毛囊细胞相似，APCDD 1对β-Catenin的表达和DFC中TCF/LEF报告基因测定的活性至关重要。此外，APCDD 1的缺失抑制了成骨标志物如RUNX 2的表达，并降低了基质矿化。然而，与先前研究中的毛囊细胞类似，口腔鳞状癌细胞的对照细胞培养物显示APCDD 1抑制β-连环蛋白和经典WNT/β-连环蛋白途径的典型靶基因的表达。总之，我们的数据揭示了APCDD 1在成骨细胞成骨分化过程中的不寻常的作用。APCDD 1维持β-连环蛋白的表达和活化。
In hair follicle cells APCDD1 inhibits the canonical WNT/β-Catenin pathway and its inactivation is associated with an autosomal dominant form of hair loss. We analyzed the role of APCDD1 for the osteogenic differentiation in dental follicle cells (DFCs) and identified a new and surprising function. Contrarily to hair follicle cells APCDD1 was crucial for the expression of β-Catenin and for the activity of the TCF/LEF reporter assay in DFCs. In addition, a depletion of APCDD1 inhibits the expression of osteogenic markers such as RUNX2 and decreased the matrix mineralization. However, similar to hair follicle cells in previous studies a control cell culture with oral squamous carcinoma cells showed that APCDD1 inhibits the expression of β-Catenin and of typical target genes of the canonical WNT/β-Catenin pathway. In conclusion, our data disclosed an unusual role of APCDD1 in DFCs during the osteogenic differentiation. APCDD1 sustains the expression and activation of β-Catenin.