New perspectives on the diversification of the RNA interference system: insights from comparative genomics and small RNA sequencing.
New perspectives on the diversification of the RNA interference system: insights from comparative genomics and small RNA sequencing.
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DOI：
10.1002/wrna.1210
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发表时间：
2014-03
影响因子：
7.3
通讯作者：
Aravind, L.
中科院分区：
文献类型：
--
作者：
Burroughs, Alexander Maxwell;Ando, Yoshinari;Aravind, L.
由于最近将深层序列技术应用于各种真核生物的小RNA，我们对调节多种生物过程的普遍参与的理解已大大增加了。我们回顾了当前著名的小RNA类别，并将其放置在RNAi蛋白质机械的重建进化史的背景下。该综合表明真核RNAi系统的最早版本可能利用了三种类型的前体处理的小RNA：1）感官抗态度的转录产物，2）基因组编码的，基因组编码的，不完美的汇总发夹序列，3）较大的非编码RNA前体前体前体前体前体前体序列序列。 PIWI蛋白的结构性解剖以及最近发现的新家族（包括介体复合物的Med13）表明，具有N末端结构域（也分别融合到原核酶中的邻苯二个酶）的出现通过祖先单位的重复形成是它们作为主要RNAi效应子的募集的关键，并且使用了某些首选长度的小RNA。原核PIWI蛋白通常是几个RNA指导DNA限制或CRISPR/CAS系统的组成部分。然而，真核版本似乎是从演变为RNA指导RNA干扰的子集中出现的。它们是与RNASEIII结构域和RDRP结构域一起招募的，也从原核系统中招募，形成了核心真核生物RNAi系统。像某些调节系统一样，RNAi与真核核细胞质隔室化的两个不同但连接的臂伴随着。随后的RNAi阐述是通过核心蛋白机制的多样化，通过谱系特异性扩展以及从原核生物（核酸酶和小的RNA修饰酶）募集的新成分，从而允许缔合小RNA的多样化。
Our understanding of the pervasive involvement of small RNAs in regulating diverse biological processes has been greatly augmented by recent application of deep-sequencing technologies to small RNA across diverse eukaryotes. We review the currently-known small RNA classes and place them in context of the reconstructed evolutionary history of the RNAi protein machinery. This synthesis indicates the earliest versions of eukaryotic RNAi systems likely utilized small RNA processed from three types of precursors: 1) sense-antisense transcriptional products, 2) genome-encoded, imperfectly-complementary hairpin sequences, and 3) larger non-coding RNA precursor sequences. Structural dissection of PIWI proteins along with recent discovery of novel families (including Med13 of the Mediator complex) suggest that emergence of a distinct architecture with the N-terminal domains (also occurring separately fused to endoDNases in prokaryotes) formed via duplication of an ancestral unit was key to their recruitment as primary RNAi effectors and use of small RNAs of certain preferred lengths. Prokaryotic PIWI proteins are typically components of several RNA-directed DNA restriction or CRISPR/Cas systems. However, eukaryotic versions appear to have emerged from a subset that evolved RNA-directed RNA interference. They were recruited alongside RNaseIII domains and RdRP domains, also from prokaryotic systems, to form the core eukaryotic RNAi system. Like certain regulatory systems, RNAi diversified into two distinct but linked arms concomitant with eukaryotic nucleo-cytoplasmic compartmentalization. Subsequent elaboration of RNAi proceeded via diversification of the core protein machinery through lineage-specific expansions and recruitment of new components from prokaryotes (nucleases and small RNA-modifying enzymes), allowing for diversification of associating small RNAs.
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影响因子：
4.5
作者：
Avesson, Lotta;Reimegard, Johan;Soderbom, Fredrik
通讯作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
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作者：
Aphasizhev, R;Aphasizheva, I;Simpson, L
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影响因子：
4.5
作者：
Aphasizheva, Inna;Ringpis, Gene-Errol;Aphasizhev, Ruslan
通讯作者：
Aphasizhev, Ruslan
DOI：
10.1073/pnas.0800334105
发表时间：
2008-06-10
影响因子：
11.1
作者：
Azuma-Mukai, Asuka;Oguri, Hideo;Siomi, Mikiko C.
通讯作者：
Siomi, Mikiko C.