Stimulation of intestinal cytokinetics and mucin turnover in rats fed wheat bran or cellulose.
Stimulation of intestinal cytokinetics and mucin turnover in rats fed wheat bran or cellulose.
复制标题
刺激喂食麦麸或纤维素的大鼠的肠道细胞因子和粘蛋白周转。
DOI：
10.1093/ajcn/41.5.895
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
M. Cassidy
中科院分区：
文献类型：
--
作者：
G. Vahouny;T. Le;I. Ifrim;S. Satchithanandam;M. Cassidy
大鼠分别饲喂不含纤维、10%麦麸或10%纤维素的饲料，并分别通过光镜和放射自显影术评估肠道形态和细胞动力学。在以麸皮喂养的动物中，空肠的形态外观、细胞/绒毛柱的数量或杯状细胞的数量与对照组相比没有差异。然而，胸苷激酶[3H]后1和24小时的放射自显像分析显示肠细胞的周转和绒毛转运增加。标记硫酸盐掺入也增加了2.5倍，[3H]葡萄糖掺入总肠糖蛋白和粘蛋白增加了2倍。同样的，尽管没有那么显著的结果，在喂食含有纤维素的食物的老鼠身上也得到了。这些研究提供了含有某些纤维衍生物的饮食可以改变肠细胞更新的证据，并支持了早期形态学观察结果，即喂养这些纤维衍生物会增加杯状细胞的分泌活性。
Rats were fed defined diets containing no fiber, 10% wheat bran or 10% cellulose, and intestinal morphology and cytokinetics were assessed by light microscopy and autoradiography, respectively. In bran-fed animals, there were no differences in morphological appearance of the jejunum, in the number of cells/villus column or in numbers of goblet cells compared to controls. Autoradiographic analysis, at one and 24 h after [3H]thymidine, however, suggested an increased turnover and villus transit of intestinal cells. There was also a 2.5 fold increase in incorporation of labeled sulfate, and a 2-fold increase in [3H]glucose incorporation into total intestinal glycoproteins and mucins. Similar, albeit less dramatic results were obtained in rats fed diets containing cellulose. These studies provide evidence that diets containing certain fiber derivatives can alter aspects of intestinal cell turnover, and support the earlier morphological observations suggesting increased goblet cell secretory activity in response to feeding these fiber derivatives.