Lumbricus extract promotes the regeneration of injured peripheral nerve in rats
Lumbricus extract promotes the regeneration of injured peripheral nerve in rats
复制标题
DOI：
10.1016/j.jep.2009.02.030
复制
发表时间：
2009-05-04
影响因子：
5.4
通讯作者：
Jiang, Baoguo
中科院分区：
文献类型：
--
作者：
Wei, Shaoyin;Yin, Xiaofeng;Jiang, Baoguo
背景：如果神经系统完好，蚯蚓可以再生身体的截肢部分。地龙是一种传统中药，在中国被用于促进神经功能已有数百年的历史。本研究的目的：探讨地龙提取物对大鼠周围神经再生的有益作用。假手术组（手术但不夹闭坐骨神经）、对照组和治疗组分别用2 ml 0.9%NaCl、0.9%NaCl和地龙提取物（1g/ml）处理。术后每天给药一次，持续6周。在此期间，通过步行轨迹分析监测运动功能，通过电生理学监测损伤坐骨神经的传导功能，通过免疫组化评价有髓神经的再生情况。(2)损伤坐骨神经传导速度，治疗组在第3周和第6周均高于对照组。(3)在再生的有髓神经纤维数量方面，治疗组在第2周和第6周均高于对照组。结论：地龙提取物可促进坐骨神经损伤后的再生和功能恢复，提示地龙提取物在治疗人类周围神经损伤方面具有临床应用潜力。(C)2009爱思唯尔爱尔兰有限公司保留所有权利。
Background: Earthworms regenerate amputated parts of their body if the nervous system is intact. Lumbricus is one traditional Chinese medicine (TCM), which has been used in China to promote nerve function for hundreds of years.Aim of the study: To investigate the beneficial effect of lumbricus extract on peripheral nerve regeneration in rats.Materials and methods: Nerve function was surgically impaired in Sprague-Dawley (SD) rats by clamping of the left sciatic nerve. The sham-operated group (surgery but no sciatic nerve clamping), control group, and treatment group were treated with 2 ml 0.9% NaCl, 0.9% NaCl, and lumbricus extract (1 g/ml), respectively. Treatments were administered once daily after the operation for 6 weeks. During this period, motor function was monitored by walking track analysis, conduction function of injured sciatic nerve was monitored by electrophysiology, and regeneration of myelinated nerve was assessed by immunohistochemistry.Results: (1) For nerve function index value, treatment group is higher than control group. (2) For conduction velocity of injured sciatic nerve, treatment group is higher than control group at week 3 and 6. (3) For the number of regenerated myelinated nerve fibers, treatment group is higher than control group at week 2 and 6.Conclusions: Lumbricus extract appears to enhance sciatic nerve regeneration and function recovery following injury, suggesting the clinical potential of lumbricus extract on the treatment of peripheral nerve injury in humans. (C) 2009 Elsevier Ireland Ltd. All rights reserved.