Complex effects of interferon-α on the cytokine network in HIV infection -: Possible contribution to immunosuppression
Complex effects of interferon-α on the cytokine network in HIV infection -: Possible contribution to immunosuppression
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DOI：
10.1006/cyto.2000.0850
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发表时间：
2001-04-07
期刊：
影响因子：
3.8
通讯作者：
Froland, SS
中科院分区：
文献类型：
--
作者：
Stylianou, E;Aukrust, P;Froland, SS
由于白细胞介素（IL-）2、IL-10和IL-12可能参与人类免疫缺陷病毒（HIV）感染的发病机制，我们在10例HIV感染患者和10例健康对照的外周血单个核细胞（PBMC）的不同亚群培养中检测了干扰素(IFN)- α对这些细胞因子的影响。我们的主要发现是：(1) ifn - α以剂量依赖的方式显著提高了脂多糖（LPS）和植物血凝素（PHA）刺激的PBMC以及抗cd3和抗cd3 /抗cd28刺激的T细胞中IL-10水平。(2)与此相反，ifn - α对念珠菌刺激的PBMC中IL-10水平有下调作用，在hiv感染患者中抑制作用特别强。(3)此外，ifn - α对PHA和念珠菌刺激的PBMC和抗cd3刺激的T细胞中IL-2水平有显著但适度的刺激作用。(4)在lps刺激的PBMC中，ifn - α以剂量依赖的方式增强了n-12水平。我们发现ifn - α显著增强IL-10，适度增强IL-2和IL-12，表明ifn - α在hiv感染患者中具有净免疫抑制作用，可能有助于这些患者免疫缺陷的进展。(C) 2001学术出版社
Since interleukin (IL-)2, IL-10 and IL-12 may contribute to the pathogenesis of human immune deficiency virus (HIV) infection we examined the effect of interferon (IFN)-alpha on these cytokines in cultures of various subsets of peripheral blood mononuclear cells (PBMC) in ten HIV-infected patients and ten healthy controls, Our main findings were: (1) IFN-alpha markedly enhanced IL-10 levels in a dose-dependent manner in both lipopolysaccharide (LPS)- and phytohaemagglutinin (PHA)-stimulated PBMC, as well as in anti-CD3- and anti-CD3/anti-CD28-stimulated T cells in both HIV-infected patients and controls, (2) In contrast, IFN-alpha had a downregulatory effect on IL-10 levels in Candida-stimulated PBMC,with particularly strong suppressive effect in HIV-infected patients. (3) Furthermore, IFN-alpha had a significant but modest stimulatory effect on IL-2 levels in PHA- and Candida-stimulated PBMC and anti-CD3-stimulated T cells. (4) IFN-alpha enhanced n-12 levels in a dose-dependent manner in LPS-stimulated PBMC in both patients and controls. Our findings that IFN-alpha markedly enhanced IL-10 and modestly enhanced IL-2 and IL-12, suggest a net immunosuppressive effect of IFN-alpha in HIV-infected patients, possibly contributing to progression of immunodeficiency in these patients. (C) 2001 Academic Press