Effect of hydrazine based deproteination protocol on bone mineral crystal structure
Effect of hydrazine based deproteination protocol on bone mineral crystal structure
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DOI：
10.1007/s10856-012-4593-7
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发表时间：
2012-05-01
影响因子：
3.7
通讯作者：
Kontoyannis, C. G.
中科院分区：
文献类型：
--
作者：
Karampas, I. A.;Orkoula, M. G.;Kontoyannis, C. G.
在几种骨脱蛋白方案中，用于蛋白质裂解的化学剂是联氨。研究了肼脱蛋白方法对骨矿物晶体大小和结晶度的影响。牛骨采用该方法，剩余骨矿物的晶体大小和结晶度通过x射线衍射（XRD），通过测量（002）反射最大强度的一半处的宽度来确定。结果表明，肼脱蛋白可显著提高晶体尺寸和结晶度。将联氨温度从25℃提高到55℃，效果增强。红外光谱分析表明，联氨有利于骨矿物中碳酸盐和酸性磷酸盐离子的去除。提出晶体尺寸和结晶度的改变机理在于这些离子的去除，从而引起晶体的重组。
In several bone deproteination protocols the chemical agent used for protein cleavage is hydrazine. The effect of hydrazine deproteination method on the crystal size and crystallinity of the bone mineral was studied. Bovine bones were subjected to this protocol and the crystal size and crystallinity of the remaining bone mineral were determined by X-ray Diffraction (XRD), by measuring the width at the half of the maximum intensity of the (002) reflection. It was found that hydrazine deproteination induces noteworthy increase of crystal size and crystallinity. The effect was enhanced by increasing hydrazine temperature from 25 to 55A degrees C. Furthermore, infrared spectroscopy revealed that hydrazine facilitates the removal of carbonate and acid phosphate ions from bone mineral. It is proposed that the mechanism of modification of crystal size and crystallinity lies on the removal of these ions thus, resulting in crystal re-organization.