Molecular, physiological and pathogenic variability of Pyrenochaeta lycopersici associated with corky rot disease of tomato plants in Turkey
Molecular, physiological and pathogenic variability of Pyrenochaeta lycopersici associated with corky rot disease of tomato plants in Turkey
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DOI：
10.1007/s12600-011-0150-z
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发表时间：
2011-04-01
期刊：
影响因子：
1.4
通讯作者：
Oksal, Ercin
中科院分区：
文献类型：
--
作者：
Bayraktar, Harun;Oksal, Ercin
分离的Pyrenochaeta lycopersici，对番茄植物的软木根腐病的病原体，进行了评估的生理和遗传特性，使用常规和分子技术。所有菌株均能在番茄根上产生坏死，并根据最适生长温度将其分为温度组。特异性PCR检测和核糖体DNA（rDNA）的内部转录间隔区的DNA序列分析证实存在两种类型（1型和2型）的病原体之间的测试的分离株。除分离株P1 - 4归类为1型外，所有分离株均被鉴定为2型。限制性片段长度多态性（RFLP）分析与六种酶导致三个不同的带型之间的分离株取决于rDNA基因间间隔区的长度和限制性图谱。简单重复序列分析表明，菌株间存在较高的遗传多样性，与RFLP分析结果一致。这些结果表明，番茄疫霉土耳其分离物中存在3个不同的种内类群。本研究是第一次尝试描述土耳其番茄疫霉分离株的特征。本研究结果可为番茄抗疫苗的筛选提供参考。
Isolates of Pyrenochaeta lycopersici, the causal agent of corky root rot on tomato plants, were assessed for physiological and genetic characteristics using conventional and molecular techniques. All isolates were able to produce necrosis on tomato roots and classified into temperature group according to the optimal growth temperatures. Specific-PCR assays and DNA sequence analysis of the ribosomal DNA (rDNA) internal transcribed spacer region confirmed the existence of both types (Type 1 and Type 2) of the pathogen among the isolates tested. All isolates were identified as Type 2 except for isolate Pl-4, which was classified as Type 1. Restriction fragment length polymorphism (RFLP) analysis with six enzymes resulted in three distinct banding patterns among the isolates depending on the length and restriction profiles of the rDNA intergenic spacer region. Inter-simple sequence-repeat analysis revealed a high level of genetic diversity among the isolates in agreement with the data of RFLP analysis. These results indicated that there were three different intraspecific groups among Turkish isolates of P. lycopersici. The presented study is the first attempting to characterize Turkish isolates of P. lycopersici. The results obtained will be useful in screening of tomato seedlings for resistance to P. lycopersici.