Gel-free/label-free proteomic analysis of root tip of soybean over time under flooding and drought stresses.
Gel-free/label-free proteomic analysis of root tip of soybean over time under flooding and drought stresses.
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DOI：
10.1016/j.jprot.2015.09.007
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发表时间：
2016
影响因子：
3.3
通讯作者：
Xin Wang;M. Oh;K. Sakata;S. Komatsu
中科院分区：
文献类型：
--
作者：
Xin Wang;M. Oh;K. Sakata;S. Komatsu
洪涝和干旱胁迫对大豆早期生长有明显抑制作用。为探讨大豆对水涝和干旱胁迫的响应机制，采用无凝胶/无标记蛋白质组学技术对大豆根尖的时间蛋白谱进行了分析。根尖是抗涝最敏感的器官，在干旱条件下有利于根系渗透。大豆根系中糖蛋白葡萄糖基转移酶（UDP glucose: glycoprotein glucosyltransferase）在洪涝和干旱条件下分别降低和升高。时间蛋白质谱表明，在洪涝和干旱条件下，根尖的发酵和蛋白质合成/降解分别是必需的。硅蛋白-蛋白互作分析表明，s -腺苷蛋氨酸合成酶家族蛋白与B-S葡萄糖苷酶44在洪涝和干旱条件下分别具有诱导和抑制作用，其相互作用与碳水化合物代谢有关。此外，生物素/脂酰附着结构域蛋白和II类氨基酰基tRNA/生物素合成酶超家族蛋白在时间胁迫下在根尖受到抑制。这些结果表明，生物素和生物素化可能参与大豆根尖早期应对洪涝和干旱的能量管理。
Growth in the early stage of soybean is markedly inhibited under flooding and drought stresses. To explore the responsive mechanisms of soybean, temporal protein profiles of root tip under flooding and drought stresses were analyzed using gel-free/label-free proteomic technique. Root tip was analyzed because it was the most sensitive organ against flooding, and it was beneficial to root penetration under drought. UDP glucose: glycoprotein glucosyltransferase was decreased and increased in soybean root under flooding and drought, respectively. Temporal protein profiles indicated that fermentation and protein synthesis/degradation were essential in root tip under flooding and drought, respectively.In silicoprotein–protein interaction analysis revealed that the inductive and suppressive interactions betweenS-adenosylmethionine synthetase family protein and B-S glucosidase 44 under flooding and drought, respectively, which are related to carbohydrate metabolism. Furthermore, biotin/lipoyl attachment domain containing protein and Class II aminoacyl tRNA/biotin synthetases superfamily protein were repressed in the root tip during time-course stresses. These results suggest that biotin and biotinylation might be involved in energy management to cope with flooding and drought in early stage of soybean-root tip.