LATE MERISTEM IDENTITY1 regulates leaf margin development via the auxin transporter gene SMOOTH LEAF MARGIN1
LATE MERISTEM IDENTITY1 regulates leaf margin development via the auxin transporter gene SMOOTH LEAF MARGIN1
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LATE MERISTEM IDENTITY1 通过生长素转运蛋白基因 SMOOTH LEAF MARGIN1 调节叶缘发育
DOI：
10.1093/plphys/kiab268
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发表时间：
2021
期刊：
影响因子：
7.4
通讯作者：
Chuanen Zhou
中科院分区：
文献类型：
--
作者：
Xiao Wang;Zhang Juanjuan;Yangyang Xie;Xiu Liu;Lizhu Wen;Hongfeng Wang;Jing Zhang;Jie Li;Lu Han;Xiaolin Yu;Kirankumar S. Mysore;Jiangqi Wen;Chuanen Zhou
摘要植物叶片具有多种形态，LMI 1及其同源基因编码的同源结构域亮氨酸拉链转录因子是植物叶片发育调控的进化热点。然而，LMI 1介导的调控机制的叶片形状的形成在很大程度上是未知的。MtLMI 1a和MtLMI 1b是模式豆科植物桶形苜蓿（Medicago truncatula）中LMI 1的推定直向同源物。在这里，我们调查的作用MtLMI 1a和MtLMI 1b在叶缘形态发生的功能丧失突变体的特点。MtLMI 1a和MtLMI 1b沿叶缘沿着以接近互补的模式表达，并且它们冗余地促进叶缘锯齿的发育，如在它们的双突变体中相对平滑的叶缘所揭示的。此外，MtLMI 1直接激活光滑叶缘1（SLM 1）的表达，其编码生长素流出载体，从而调节生长素沿叶缘的沿着分布。进一步的分析表明，MtLMI 1基因与无顶端分生组织（MtNAM）和Argonaute 7（MtAGO 7）介导的反式作用短干扰RNA 3（TAS 3 ta-siRNA）途径相互作用，以形成最终的叶缘形状。MtLMI 1 s参与生长素依赖的叶缘形成是有趣的功能保护的背景下。此外，LMI 1 s及其推定的旁系同源物在关键领域的不同表达模式是功能特化的重要驱动因素，尽管它们在物种之间的功能等同。
Abstract Plant leaves have evolved into diverse shapes and LATE MERISTEM IDENTITY1 (LMI1) and its putative paralogous genes encode homeodomain leucine zipper transcription factors that are proposed evolutionary hotspots for the regulation of leaf development in plants. However, the LMI1-mediated regulatory mechanism underlying leaf shape formation is largely unknown. MtLMI1a and MtLMI1b are putative orthologs of LMI1 in the model legume barrelclover (Medicago truncatula). Here, we investigated the role of MtLMI1a and MtLMI1b in leaf margin morphogenesis by characterizing loss-of-function mutants. MtLMI1a and MtLMI1b are expressed along leaf margin in a near-complementary pattern, and they redundantly promote development of leaf margin serrations, as revealed by the relatively smooth leaf margin in their double mutants. Moreover, MtLMI1s directly activate expression of SMOOTH LEAF MARGIN1 (SLM1), which encodes an auxin efflux carrier, thereby regulating auxin distribution along the leaf margin. Further analysis indicates that MtLMI1s genetically interact with NO APICAL MERISTEM (MtNAM) and the ARGONAUTE7 (MtAGO7)-mediated trans-acting short interfering RNA3 (TAS3 ta-siRNA) pathway to develop the final leaf margin shape. The participation of MtLMI1s in auxin-dependent leaf margin formation is interesting in the context of functional conservation. Furthermore, the diverse expression patterns of LMI1s and their putative paralogs within key domains are important drivers for functional specialization, despite their functional equivalency among species.