Contribution of actin filaments and microtubules to quasi-in situ tensile properties and internal force balance of cultured smooth muscle cells on a substrate
Contribution of actin filaments and microtubules to quasi-in situ tensile properties and internal force balance of cultured smooth muscle cells on a substrate
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DOI：
10.1152/ajpcell.00098.2008
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发表时间：
2008-12-01
影响因子：
5.5
通讯作者：
Matsumoto, Takeo
中科院分区：
文献类型：
--
作者：
Nagayama, Kazuaki;Matsumoto, Takeo
Nagayama K，MatSumoto T.肌动蛋白细丝和微管对基质上培养的平滑肌细胞准原位拉伸特性和内力平衡的贡献。Am J Physiol Cell Physiol 295：C1569-C1578,2008。2008年10月15日首次出版；DOI：10.1152/ajpcell.00098.2008。-研究了肌动蛋白细丝(AFs)和微管(MT)对培养的大鼠主动脉平滑肌细胞(SMC)准原位拉伸性能和细胞内力平衡的影响。用一对微吸管固定培养在基质上的SMC，在保持原位细胞形态和细胞骨架完整性的情况下逐渐从基质上分离，然后以每5 S 1微米的速度拉伸至约15%并卸载3次。未处理病例的细胞硬度接近20NN/%应变，AF和MT破坏后细胞硬度分别下降约65%和30%。MT增强对细胞硬度无明显影响。AFS和MTS在抵抗细胞伸展和缩短方面的作用是通过非侵入性脱离热敏明胶涂层培养皿的细胞面积回缩来评估的。在未经处理的细胞中，回缩程度类似于40%，而在房颤破坏的细胞中，这一比例为
Nagayama K, Matsumoto T. Contribution of actin filaments and microtubules to quasi-in situ tensile properties and internal force balance of cultured smooth muscle cells on a substrate. Am J Physiol Cell Physiol 295: C1569-C1578, 2008. First published October 15, 2008; doi:10.1152/ajpcell.00098.2008.-The effects of actin filaments (AFs) and microtubules (MTs) on quasi-in situ tensile properties and intracellular force balance were studied in cultured rat aortic smooth muscle cells (SMCs). A SMC cultured on substrates was held using a pair of micropipettes, gradually detached from the substrate while maintaining in situ cell shape and cytoskeletal integrity, and then stretched up to similar to 15% and unloaded three times at the rate of 1 mu m every 5 s. Cell stiffness was similar to 20 nN per percent strain in the untreated case and decreased by similar to 65% and similar to 30% following AF and MT disruption, respectively. MT augmentation did not affect cell stiffness significantly. The roles of AFs and MTs in resisting cell stretching and shortening were assessed using the area retraction of the cell upon noninvasive detachment from thermoresponsive gelatin-coated dishes. The retraction was similar to 40% in untreated cells, while in AF-disrupted cells it was