Novel monoclonal antibodies against major antigens of Mycobacterium bovis.
Novel monoclonal antibodies against major antigens of Mycobacterium bovis.
复制标题
针对牛分枝杆菌主要抗原的新型单克隆抗体。
DOI：
10.1046/j.1365-3083.2001.00914.x
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发表时间：
2001
影响因子：
3.7
通讯作者：
Pollock,JM
中科院分区：
文献类型：
--
作者：
Lyashchenko,KP;Wiker,HG;Harboe,M;McNair,J;Komissarenko,SV;Pollock,JM
MPB70 和 MPB83 是定义牛分枝杆菌强表达表型的最具特征性的输出蛋白之一。已知这些蛋白质与成骨细胞特异性因子 2 同源。通过分枝杆菌的体外培养，它们似乎具有有限的物种分布，并且对分枝杆菌具有相对特异性。然而，结核分枝杆菌中存在几乎相同的基因。为了促进进一步研究这些蛋白质的免疫生物学及其在结核分枝杆菌所致结核病鉴别诊断中的潜在应用。针对牛，我们描述了 20 种单克隆抗体 (MoAb) 对这些蛋白质的反应性。用牛 PPD 免疫产生 10 个 MoAb。这些抗体在 SDS-PAGE 和蛋白质印迹中使用还原条件优先与可溶性 MPB70 抗原反应。通过使用源自 M 的全细胞声波提取物的级分对小鼠进行免疫接种，产生了 10 种 MoAb。牛。这些抗体优先与表面暴露的 MPB83 脂糖蛋白反应。
MPB70 and MPB83 are among the most characteristically exported proteins defining a strongly expressed phenotype ofMycobacterium bovis. These proteins are known to be homologous to osteoblast‐specific factor 2. Byin vitroculture of mycobacteria they appear to have a limited species distribution and to be relatively specific forM. bovis.Virtually identical genes are however, present inMycobacterium tuberculosis. In order to facilitate further research into the immunobiology of these proteins and their potential application for differential diagnosis of tuberculosis as a result ofM. bovis, we describe the reactivities of 20 monoclonal antibodies (MoAbs) to these proteins. Immunizing with bovine PPD generated 10 MoAbs. These antibodies reacted preferentially with the soluble MPB70 antigen using reducing conditions in SDS‐PAGE with western blotting. Ten MoAbs were generated by immunizing mice with fractions derived from a whole cell sonic extract ofM. bovis. These antibodies reacted preferentially with the surface exposed MPB83 lipoglycoprotein.