Xylitol production at high temperature by engineered Kluyveromyces marxianus.
Xylitol production at high temperature by engineered Kluyveromyces marxianus.
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DOI：
10.1016/j.biortech.2013.10.109
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发表时间：
2014
影响因子：
11.4
通讯作者：
Jia Zhang;Biao Zhang;Dongmei Wang;Xiaolian Gao;Jiong Hong
中科院分区：
文献类型：
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作者：
Jia Zhang;Biao Zhang;Dongmei Wang;Xiaolian Gao;Jiong Hong
通过过量表达粗糙脉孢菌木糖还原酶基因构建了几株重组马克斯克鲁维酵母。YZJ015保持了原始的木糖醇脱氢酶基因，在42 °C下从100 g L− 1木糖中以最高的产率（1.49 g L−1h−1）生产木糖醇。即使在45 °C下，YZJ015仍然能够从100 g L− 1木糖生产60.03 g L − 1木糖醇，生产率为1.25 g L−1h−1。此外，对于42 °C下的20轮细胞循环，YZJ015从100 g L− 1木糖生产71.35 g L− 1木糖醇，每个循环的生产率为4.43 g L−1h− 1。木糖醇脱氢酶基因被破坏的YZJ017在42 °C下从100 g L− 1木糖和40 g L− 1甘油作为共底物以1.01 g g− 1的产量产生100.02 g L − 1木糖醇。这些工程菌株为在高温下生产木糖醇提供了良好的基础。
Several recombinantKluyveromyces marxianusstrains were constructed through overexpressing theNeurospora crassaxylose reductase genes. YZJ015, which maintained the original xylitol dehydrogenase gene, produced xylitol with the highest productivity (1.49 g L−1h−1) from 100 g L−1xylose at 42 °C. Even at 45 °C, YZJ015 was still able to produce 60.03 g L−1xylitol from 100 g L−1xylose with a productivity of 1.25 g L−1h−1. In addition, for 20 rounds of cell recycling at 42 °C, YZJ015 produced 71.35 g L−1xylitol from 100 g L−1xylose with a productivity of 4.43 g L−1h−1per cycle. YZJ017, in which the xylitol dehydrogenase gene was disrupted, produced 100.02 g L−1xylitol at a yield of 1.01 g g−1from 100 g L−1xylose with 40 g L−1glycerol as co-substrate at 42 °C. These engineered strains provide an excellent foundation for xylitol production at elevated temperatures.