MAPPING OF VSG GENES ON LARGE EXPRESSION-SITE CHROMOSOMES OF TRYPANOSOMA-BRUCEI SEPARATED BY PULSED-FIELD GRADIENT ELECTROPHORESIS
MAPPING OF VSG GENES ON LARGE EXPRESSION-SITE CHROMOSOMES OF TRYPANOSOMA-BRUCEI SEPARATED BY PULSED-FIELD GRADIENT ELECTROPHORESIS
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DOI：
10.1016/0378-1119(86)90209-x
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发表时间：
1986-01-01
期刊：
影响因子：
3.5
通讯作者：
BORST, P
中科院分区：
文献类型：
--
作者：
JOHNSON, PJ;BORST, P
我们对布鲁氏锥虫(品系427)的脉冲梯度电泳法条件进行了改进，将先前迁徙的大染色体分解为五条不同的染色体带。对4个不同的变异表面糖蛋白(VSG)基因的定位表明，在感染后期的8个独立的VSG基因切换事件中，激活的基因总是易位到同一染色体带。此外，我们还检测了两个VSG基因的染色体位置，这两个基因在感染早期被激活而没有复制。每一个的位置在其活动和非活动状态下是恒定的。这些数据确定了一条染色体带，该带在该锥虫株慢性感染期间优先用于VSG基因的表达，并支持VSG基因的转录和易位受到独立调控的概念。
We have modified the conditions for pulsed-field gradient electrophoresis of Trypanosoma brucei (strain 427) to resolve large chromosomes, which previously comigrated, into five distinct chromosome bands. Mapping of four different variant surface glycoprotein (VSG) genes, which were duplicatively activated in eight independent VSG gene switching events during later stages of infection, has shown that the activated gene is invariably translocated to the same chromosome band. Also, we have examined the chromosomal location of two VSG genes which were activated without duplication during early infection. The location of each is constant in its active and inactive state. These data identify a single chromosome band which is preferentially used for VSG gene expression during chronic infections in this trypanosome strain and support the concept that the transcription and the translocation of VSG genes are independently regulated.