Regulation of Drosophila yolk protein genes by an ovary-specific GATA factor.
Regulation of Drosophila yolk protein genes by an ovary-specific GATA factor.
复制标题
卵巢特异性 GATA 因子对果蝇卵黄蛋白基因的调节。
DOI：
10.1128/mcb.15.12.6943
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发表时间：
1995
影响因子：
5.3
通讯作者：
Wensink,PC
中科院分区：
文献类型：
--
作者：
Lossky,M;Wensink,PC
黑腹果蝇的分化转录蛋白（yp1和yp2）仅在成年雌性、脂肪体组织和卵巢卵泡细胞中表达。通过体外转录实验，我们发现了一个12bp的DNA元件，可以激活这两个ypgenes的启动子的转录。在体内，这种调节元件是组织特异性的：它激活卵泡细胞中yp1和yp2报告基因的转录，但在脂肪体或其他组织中没有可检测到的作用。该元件的序列由GATA转录因子家族的两个识别位点组成。我们发现，在已知编码GATA因子的果蝇中，只有GATA因子在卵巢中表达。我们在卵巢中检测到的单一转录本被选择性地剪接或启动，以产生卵巢特异性的蛋白质异构体。细菌表达的dGATAb与12bp元件结合；类似的结合活性也存在于用于体外转录测定的Kc0核提取物中。这些体外和体内的结果使我们提出dgatabp产生几种发育调节产物，其中之一是卵泡细胞特异性蛋白，从已知的调节元件激活yp1和yp2的转录。
The divergently transcribedyolk proteingenes (Yp1andYp2) ofDrosophila melanogasterare expressed only in adult females, in fat body tissue and in ovarian follicle cells. Using an in vitro transcription assay, we have identified a single 12-bp DNA element that activates transcription from the promoters of bothYpgenes. In vivo, this regulatory element is tissue specific: it activates transcription ofYp1andYp2reporter genes in follicle cells but has no detectable effect in fat body or other tissues. The sequence of the element consists of two recognition sites for the GATA family of transcription factors. We show that among theDrosophilagenes known to encode GATA factors, onlydGATAbis expressed in ovaries. The single transcript that we detect in ovaries is alternatively spliced or initiated to produce an ovary-specific isoform of the protein. Bacterially expressed dGATAb binds to the 12-bp element; a similar binding activity is also present in the Kc0 nuclear extracts used for in vitro transcription assays. These in vitro and in vivo results lead us to propose thatdGATAbmakes several developmentally regulated products, one of which is a follicle cell-specific protein activating transcription ofYp1andYp2from a known regulatory element.