SlDEAD31, a Putative DEAD-Box RNA Helicase Gene, Regulates Salt and Drought Tolerance and Stress-Related Genes in Tomato.
SlDEAD31, a Putative DEAD-Box RNA Helicase Gene, Regulates Salt and Drought Tolerance and Stress-Related Genes in Tomato.
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SlDEAD31，一种假定的 DEAD-Box RNA 解旋酶基因，调节番茄的耐盐性、耐旱性以及胁迫相关基因
DOI：
10.1371/journal.pone.0133849
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Hu Z
中科院分区：
文献类型：
--
作者：
Zhu M;Chen G;Dong T;Wang L;Zhang J;Zhao Z;Hu Z
DEAD-box RNA解旋酶参与RNA代谢的几乎每个方面，与包括植物生长和发育在内的多种细胞功能相关，并且它们在响应生物和非生物胁迫中的重要性才刚刚开始显现。然而，到目前为止，还没有一个DEAD盒基因在番茄中得到很好的表征。在这项研究中，我们报告了两个推定的死亡盒RNA解旋酶基因，SlDEAD 30和SlDEAD 31，这是被归类为压力相关的死亡盒蛋白的系统发育分析的鉴定和特征。表达分析表明，SlDEAD 30在根和成熟叶片中高表达，而SlDEAD 31在各种组织中稳定表达。此外，这两个基因的表达诱导主要在根在NaCl胁迫下，和SlDEAD 31 mRNA也增加了热，冷，和脱水。在胁迫试验中，过表达SlDEAD 31的转基因番茄植株表现出显著增强的耐盐性和轻微改善的抗旱性，这同时通过与野生型植株相比显著增强的多个生物和非生物胁迫相关基因的表达、更高的存活率、相对含水量（RWC）和叶绿素含量以及更低的失水率和丙二醛（MDA）产生来证明。总的来说，这些结果提供了番茄中SlDEAD 30和SlDEAD 31基因的初步表征，并表明胁迫响应性SlDEAD 31是植物耐盐和耐旱性以及胁迫相关基因调控所必需的。
The DEAD-box RNA helicases are involved in almost every aspect of RNA metabolism, associated with diverse cellular functions including plant growth and development, and their importance in response to biotic and abiotic stresses is only beginning to emerge. However, none of DEAD-box genes was well characterized in tomato so far. In this study, we reported on the identification and characterization of two putative DEAD-box RNA helicase genes, SlDEAD30 and SlDEAD31 from tomato, which were classified into stress-related DEAD-box proteins by phylogenetic analysis. Expression analysis indicated that SlDEAD30 was highly expressed in roots and mature leaves, while SlDEAD31 was constantly expressed in various tissues. Furthermore, the expression of both genes was induced mainly in roots under NaCl stress, and SlDEAD31 mRNA was also increased by heat, cold, and dehydration. In stress assays, transgenic tomato plants overexpressing SlDEAD31 exhibited dramatically enhanced salt tolerance and slightly improved drought resistance, which were simultaneously demonstrated by significantly enhanced expression of multiple biotic and abiotic stress-related genes, higher survival rate, relative water content (RWC) and chlorophyll content, and lower water loss rate and malondialdehyde (MDA) production compared to wild-type plants. Collectively, these results provide a preliminary characterization of SlDEAD30 and SlDEAD31 genes in tomato, and suggest that stress-responsive SlDEAD31 is essential for salt and drought tolerance and stress-related gene regulation in plants.