Veillonella nakazawae sp. nov., an anaerobic Gram-negative coccus isolated from the oral cavity of Japanese children
Veillonella nakazawae sp. nov., an anaerobic Gram-negative coccus isolated from the oral cavity of Japanese children
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DOI：
10.1099/ijsem.0.004583
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发表时间：
2021-01-01
影响因子：
2.8
通讯作者：
Kiyoura, Yusuke
中科院分区：
文献类型：
--
作者：
Mashima, Izumi;Theodorea, Citra F.;Kiyoura, Yusuke
从健康日本儿童的口腔中分离出两种以前未知的革兰氏阴性球菌菌株：T1-7(T) 和 S6-16。这两种菌株表现出韦荣球菌属成员的非典型表型特征，包括过氧化氢酶的产生。对它们的 16S rRNA 基因进行测序证实它们属于韦荣球菌属。在厌氧条件下，两种菌株在含有 1% (w/v) 葡萄糖、1% (w/v) 果糖和 1% (v/v) 乳酸钠的胰蛋白酶-酵母提取物血红素培养基中产生乙酸和丙酸作为代谢终产物。 16S rRNA、dnaK、rpoB 和 gltA 基因序列的比较分析表明，这两个菌株在系统发育上是同质的，并且在韦荣球菌属内构成了一个独特的、新颖的谱系。使用部分 16S rRNA、dnaK、rpoB 和 gltA 基因序列，这两个菌株的序列与两个最密切相关的物种的典型菌株（Veillonella dispar ATCC 17748(T) 和 Veillonella fanium JCM 31738(T)）具有最高的相似性，分别为 99.9%、95.8%、96.9% 和 96.7%。此外，菌株T1-7（T）与最密切相关的物种V.infantium的模式菌株具有最高的平均核苷酸同一性（ANI）值（94.06％）。同时，菌株T1-7(T)显示出与婴儿弧菌模式菌株最高的数字DNA-DNA杂交(dDDH)值(55.5％)。根据过氧化氢酶产量、部分 dnaK、rpoB 和 gltA 序列、平均 ANI 和 dDDH 值，本研究中报道的两种菌株与之前报道的韦荣氏菌属物种进行了区分。根据这些观察，这两个菌株代表了一个新物种，其名称为 Veillonella nakazawae sp。十一月被提议。模式菌株是T1-7(T) (JCM 33966(T)=CCUG 74597(T))。
Two strains of previously unknown Gram-negative cocci, T1-7(T) and S6-16, were isolated from the oral cavity of healthy Japanese children. The two strains showed atypical phenotypic characteristics of members of the genus Veillonella, including catalase production. Sequencing of their 16S rRNA genes confirmed that they belong to genus Veillonella. Under anaerobic conditions, the two strains produced acetic acid and propionic acid as metabolic end-products in a trypticase-yeast extract?haemin medium containing 1 % (w/v) glucose, 1 % (w/v) fructose and 1 % (v/v) sodium lactate. Comparative analysis of the 16S rRNA, dnaK, rpoB and gltA gene sequences revealed that the two strains are phylogenetically homogeneous and comprise a distinct, novel lineage within the genus Veillonella. The sequences from the two strains shared the highest similarity, at 99.9, 95.8, 96.9 and 96.7 %, using the partial 16S rRNA, dnaK, rpoB and gltA gene sequences, respectively, with the type strains of the two most closely related species, Veillonella dispar ATCC 17748(T) and Veillonella infantium JCM 31738(T). Furthermore, strain T1-7(T) shared the highest average nucleotide identity (ANI) value (94.06 %) with type strain of the most closely related species, V. infantium. At the same time, strain T1-7(T) showed the highest digital DNA-DNA hybridization (dDDH) value (55.5 %) with the type strain of V. infantium. The two strains reported in this study were distinguished from the previously reported species from the genus Veillonella based on catalase production, partial dnaK, rpoB and gltA sequences, average ANI and dDDH values. Based on these observations, the two strains represent a novel species, for which the name Veillonella nakazawae sp. nov. is proposed. The type strain is T1-7(T) (JCM 33966(T)=CCUG 74597(T)).