Synthesis of RNA in Isolated Cells from Xenopus laevis Embryos
Synthesis of RNA in Isolated Cells from Xenopus laevis Embryos
复制标题
非洲爪蟾胚胎分离细胞中 RNA 的合成
作者：
K. Shiokawa;O. Nada;K. Yamana
两栖动物胚胎的繁殖特别令人感兴趣，因为最近发现RNA种类的顺序合成发生在早期发育过程中：第一个，在非常早期的阶段合成，是可溶性RNA，其次是核糖体RNA，其合成在后期占主导地位1，2。这些连续事件可以提供细胞分化的参数。然而，胚胎有一个明显的缺点：它需要很少的外源物质，除了几种无机离子，它需要直到喂养阶段。对于核酸和蛋白质合成，二氧化碳可能是唯一可以自由掺入胚胎的前体。我们已经发现，从分离的胚胎中获得的细胞在加入到培养基中时会主动掺入许多放射性前体，并像整个胚胎一样连续合成RNA物质。
INVESTIGATIONS with amphibian embryos are of particular interest because of the recent finding that a sequential synthesis of RNA species takes place during early development: the first, synthesized during very early stages, is soluble RNA, followed by ribosomal RNA, the synthesis of which predominates later1,2. These sequential events may provide a parameter of cellular differentiation. The embryo has, however, a definite disadvantage: it needs few exogenous substances except for several inorganic ions which it requires until the feeding stage. For nucleic acid and protein synthesis carbon dioxide is perhaps the only available precursor which can be incorporated freely into the embryo3. We have found that the cells obtained from dissociated embryo actively incorporate a number of radioactive precursors when these are added to the medium and synthesize RNA species sequentially as do whole embryos.