Obtaining More Accurate Signals: Spatiotemporal Imaging of Cancer Sites Enabled by a Photoactivatable Aptamer-Based Strategy
Obtaining More Accurate Signals: Spatiotemporal Imaging of Cancer Sites Enabled by a Photoactivatable Aptamer-Based Strategy
复制标题
获得更准确的信号：通过基于光激活适体的策略实现癌症部位的时空成像
DOI：
10.1021/acsami.6b07450
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发表时间：
2016
影响因子：
9.5
通讯作者：
Zhou Xiang
中科院分区：
文献类型：
--
作者：
Xiao Heng;Chen Yuqi;Yuan Erfeng;Li Wei;Jiang Zhuoran;Wei Lai;Su Haomiao;Zeng Weiwu;Gan Yunjiu;Wang Zijing;Yuan Bifeng;Qin Shanshan;Leng Xiaohua;Zhou Xin;Liu Songmei;Zhou Xiang
癌症的早期诊断对相关的癌症预防和临床治疗具有重要意义，而在分子水平上有效识别癌性肿瘤的发生部位是关键。在此，我们提出了一个通用的和有效的战略的基础上的适体识别和光活化成像的癌症诊断。这是第一次将可见光控制的光活化适体平台应用于癌症诊断。基于光活化适体的策略可以准确地检测核仁素过表达的肿瘤细胞，并可用于高选择性癌细胞筛选和组织成像。该策略适用于福尔马林固定石蜡包埋组织标本和冷冻切片。此外，光活化技术在更准确和持久的成像方面比传统荧光团的使用有了很大的进步。值得注意的是，这种策略的应用可以在组织样本分析中产生与经典的苏木素-伊红染色和免疫组织化学技术相同的准确结果。
Early cancer diagnosis is of great significance to relative cancer prevention and clinical therapy, and it is crucial to efficiently recognize cancerous tumor sites at the molecular level. Herein, we proposed a versatile and efficient strategy based on aptamer recognition and photoactivation imaging for cancer diagnosis. This is the first time that a visible light-controlled photoactivatable aptamer-based platform has been applied for cancer diagnosis. The photoactivatable aptamer-based strategy can accurately detect nucleolin-overexpressed tumor cells and can be used for highly selective cancer cell screening and tissue imaging. This strategy is available for both formalin-fixed paraffin-embedded tissue specimens and frozen sections. Moreover, the photoactivation techniques showed great progress in more accurate and persistent imaging to the use of traditional fluorophores. Significantly, the application of this strategy can produce the same accurate results in tissue specimen analysis as with classical hematoxylin–eosin staining and immunohistochemical technology.