In vivo effects of temperature on the heart and pyloric rhythms in the crab Cancer borealis
In vivo effects of temperature on the heart and pyloric rhythms in the crab Cancer borealis
复制标题
DOI：
10.1242/jeb.199190
复制
发表时间：
2019-03-01
影响因子：
2.8
通讯作者：
Marder, Eve
中科院分区：
文献类型：
--
作者：
Kushinsky, Dahlia;Morozova, Ekaterina O.;Marder, Eve
多年来，人们对甲壳类的心脏节律和幽门节律分别进行了广泛的研究。局部和荷尔蒙的神经调节和感觉输入到这些中央模式生成器电路在动物对扰动的反应中发挥着重要作用，但在体外研究中通常会丢失或移除。为了同时检测甲壳类动物心脏和幽门节律的活体运动输出，我们使用了光体积描记。在被测量的人群中(n=49)，心率频率在0.3~2.3赫兹之间。幽门节律为0.2~1.6赫兹。我们观察到心脏频率和幽门节律之间的弱相关性。在长达一小时的记录中，许多处于受控温度下的动物表现出强烈的抑制性发作，其中心脏频率降低或变得静止，幽门节律频率降低。我们在记录心脏和幽门肌肉运动的同时，通过加热或冷却生理盐水浴来测量节律对温度上升的同步反应。Q(10)、临界温度(肌肉功能受损的温度)和频率的变化被计算出来。心脏节律对高温的反应比幽门节律更强健。
The heart and pyloric rhythms of crustaceans have been studied separately and extensively over many years. Local and hormonal neuromodulation and sensory inputs into these central pattern generator circuits play a significant role in an animal's response to perturbations, but are usually lost or removed during in vitro studies. To examine simultaneously the in vivo motor output of the crustacean heart and pyloric rhythms, we used photoplethysmography. In the population measured (n=49), the heart rhythm frequency ranged from 0.3 to 2.3 Hz. The pyloric rhythm varied from 0.2 to 1.6 Hz. We observed a weak correlation between the frequencies of the heart and pyloric rhythms. During multiple hour-long recordings, many animals held at a controlled temperature showed strong inhibitory bouts in which the heart decreased in frequency or become quiescent and the pyloric rhythm decreased in frequency. We measured the simultaneous responses of the rhythms to temperature ramps by heating or cooling the saline bath while recording both the heart and pyloric muscle movements. Q(10), critical temperature (temperature at which muscle function is compromised) and changes in frequency were calculated for each of the rhythms tested. The heart rhythm was more robust to high temperature than the pyloric rhythm.