Increased expression of pp60c-src in gastric mucosa of aged rats.
Increased expression of pp60c-src in gastric mucosa of aged rats.
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老年大鼠胃粘膜中 pp60c-src 的表达增加。
DOI：
10.1093/geronj/49.3.b110
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Fligiel,SE
中科院分区：
文献类型：
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作者：
Majumdar,AP;Tureaud,J;Relan,NK;Kessel,A;Dutta,S;Hatfield,JS;Fligiel,SE
本实验观察了青年（4月龄）和老年（24月龄）fischer 344大鼠胃粘膜（泌酸腺区）细胞增殖活性与pp60c-srcin表达的关系。用5-溴-2'-脱氧尿苷（BrdU）免疫反应细胞测定的胃粘膜增殖活性，发现老年大鼠比年轻大鼠高115%（p <0.001）。这与总酪氨酸激酶（Tyr-k）活性增加47%（p <0.025）和胃粘膜中Mr40、55、60和70 kDa的4个显著蛋白条带的自磷酸化增加5 - 7倍有关。对于pp60c-srcin的Tyr-k活性也观察到类似的现象，当与来自年轻动物的相应值相比时，老年大鼠显示出高69%（p <0.025）的酶活性和该蛋白质的自磷酸化程度的5倍增加。老年大鼠胃粘膜中pp60c-src的Tyr-k活性增加可能部分是由于该蛋白质的水平较高，因为通过Western印迹分析评估的pp60c-src的相对浓度显示出比年轻动物增加2 - 5倍。此外，还发现老年大鼠胃粘膜中c-src mRNA的相对浓度比年轻动物高5 - 6倍。提示pp60c-src可能参与调节老年大鼠胃粘膜的增殖过程。
The relationship between proliferative activity and the expression of pp60c-srcin gastric mucosa (oxyntic gland area) of young (4-month) and aged (24-month) fischer 344 rats was examined. Gastric mucosal proliferative activity, as assessed by 5-bromo-2'-deoxyuridine (BrdU) immunoreactive cells, was found to be 115%(p< .001) higher in aged than in young rats. This was associated with a 47%rise (p< .025) in overall tyrosine kinase (Tyr-k) activity and a 5–7-fold increase in autophosphorylation of four prominent protein bands with Mrof 40,55,60, and 70 kDa in gastric mucosal membranes. A similar phenomenon was also observed for Tyr-k activity of pp60c-srcin that the aged rats revealed a 69%(p< .025) higher enzyme activity and a 5-fold rise in the extent of autophosphorylation of this protein when compared with the corresponding values from young animals. Increased Tyr-k activity of pp60c-srcin the gastric mucosa of aged rats could in part be due to higher levels of this protein because the relative concentration of pp60c-src, as assessed by Western blot analysis, showed a 2–5-fold increase over the young animals. In addition, the relative concentration of c-src mRNA in the gastric mucosa of aged rats was also found to be 5–6-fold higher than in young animals. We suggest that pp60c-srcmay play a role in regulating gastric mucosal proliferative processes in the gastric mucosa of aged rats.