CHARACTERIZATION OF A HIGHLY SOLUBLE COLLAGENOUS MOLECULE ISOLATED FROM CHICKEN HYALINE CARTILAGE
CHARACTERIZATION OF A HIGHLY SOLUBLE COLLAGENOUS MOLECULE ISOLATED FROM CHICKEN HYALINE CARTILAGE
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DOI：
10.1021/bi00534a002
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发表时间：
1982-01-01
期刊：
影响因子：
2.9
通讯作者：
MAYNE, R
中科院分区：
文献类型：
--
作者：
REESE, CA;WIEDEMANN, H;MAYNE, R
从有限胃酶消化的鸡透明软骨中分离到一种新的胶原分子。这种含有链间二硫键的分子被称为高相对分子质量分数或HMW。对HMW的结构提出了一个详细的模型，该模型是通过对变性后的HMW的组分进行分析，以及对旋转阴影后得到的HMW的复制品进行EM观察得出的。HMW可能是一种长度为134 nm的典型的三螺旋胶原分子，其中一条链在距三螺旋一端96 nm处被切割，而另外两条链保持不断裂。这种裂解的结果是在旋转阴影后可视化时，HMW分子中出现可识别的扭结。
A new collagenous molecule was isolated from chicken hyaline cartilage after limited pepsin digestion. This molecule, which contains interchain disulfide bonds, was called the high MW fraction or HMW. A detailed model is presented for the structure of HMW, the model being derived from analyses both of components of HMW obtained after denaturation either with or without reduction and from EM observation of replicas of HMW obtained after rotary shadowing. HMW is probably a typical, triple-helical collagen molecule of length 134 nm, in which 1 of the chains was cleaved at a distance of 96 nm from one end of the triple helix, while the other 2 chains remain uncleaved. The result of this cleavage is the appearance of a recognizable kink in molecules of HMW when visualized after rotary shadowing.