ASCORBIC-ACID SUPPRESSES NATURAL-KILLER-CELL ACTIVITY IN BUFO-MARINUS
ASCORBIC-ACID SUPPRESSES NATURAL-KILLER-CELL ACTIVITY IN BUFO-MARINUS
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DOI：
10.3354/dao009001
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发表时间：
1990-08-16
影响因子：
1.4
通讯作者：
COOPER, EL
中科院分区：
文献类型：
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作者：
GHONEUM, M;SADEK, IA;COOPER, EL
研究了抗坏血酸对蟾蜍自然杀伤（NK）细胞的影响。将外周血白细胞（PBL）与不同浓度的抗坏血酸（1 × 10 - 6）预温育1小时。103，1 ×104和1 ×105 M），表现出明显的抑制NK细胞活性（31.8至77.3%）。同样，注射抗坏血酸到背淋巴囊的蟾蜍在10毫克kg-1 d-1抑制NK细胞活性; 16.1%，1周和25.8%，3周。抗坏血酸在体内和体外抑制NK活性，而不改变效应细胞与YAC-1肿瘤靶点的结合能力。
The effect of ascorbic acid on natural killer (NK) cells in the marine toad Bufo marinus has been analyzed in vitro and in vivo. Peripheral blood leukocytes (PBL) which were pre-incubated with ascorbic acid for 1 h at different concentrations (1 .times. 103, 1 .times. 104 and 1 .times. 105 M), exhibited a significant inhibition of NK cell activity (31.8 to 77.3%). Similarly, injection of ascorbic acid into the dorsal lymph sac of toads at 10 mg kg-1 d-1 inhibited NK cell activity; 16.1% at 1 wk and 25.8% at 3 wk. Suppression of NK activity by ascorbic acid in vivo and in vitro occurs without changing the binding capacity of effector cells to YAC-1 tumor targets.