Large X-Linked Palindromes Undergo Arm-to-Arm Gene Conversion across Mus Lineages.
Large X-Linked Palindromes Undergo Arm-to-Arm Gene Conversion across Mus Lineages.
复制标题
大型 X 连锁回文在小鼠谱系中经历臂对臂基因转换。
DOI：
10.1093/molbev/msaa059
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发表时间：
2020
影响因子：
10.7
通讯作者：
Mueller,JacobL
中科院分区：
文献类型：
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作者：
Swanepoel,CallieM;Gerlinger,EmmaR;Mueller,JacobL
大（>10 kb），几乎相同（>99%核苷酸同源），在哺乳动物性染色体上丰富的回文序列。灵长类y -回文经历了高速率的臂对臂基因转换，这是一种在没有X-Y重组的情况下保持其序列完整性的机制。目前还不清楚x -回文是否会发生手臂到手臂的基因转换，如果是的话，转换的速度是多少。x -回文在女性体内可以自由重组。我们生成了高质量的molossinusus和m的序列集。spretusx -回文区，并将其与同源的m。musculusX-palindromes。我们的进化序列比较发现了x -回文臂对臂基因转换的证据，其速率与小鼠常染色体等位基因的转化率相当。musx -palindrome在物种之间携带的衍生变体多于祖先变体，这表明它们的序列正在迅速分化。我们推测，除了通过序列均质化维持基因的序列完整性外，回文臂对臂基因转换也可能促进序列的快速分化。
Large (>10 kb), nearly identical (>99% nucleotide identity), palindromic sequences are enriched on mammalian sex chromosomes. Primate Y-palindromes undergo high rates of arm-to-arm gene conversion, a proposed mechanism for maintaining their sequence integrity in the absence of X–Y recombination. It is unclear whether X-palindromes, which can freely recombine in females, undergo arm-to-arm gene conversion and, if so, at what rate. We generated high-quality sequence assemblies ofMus molossinusandM. spretusX-palindromic regions and compared them with orthologousM. musculusX-palindromes. Our evolutionary sequence comparisons find evidence of X-palindrome arm-to-arm gene conversion at rates comparable to autosomal allelic gene conversion rates in mice.MusX-palindromes also carry more derived than ancestral variants between species, suggesting that their sequence is rapidly diverging. We speculate that in addition to maintaining genes’ sequence integrity via sequence homogenization, palindrome arm-to-arm gene conversion may also facilitate rapid sequence divergence.